氨磷汀与峰龄多糖对外周血造血干细胞的刺激增殖及协同作用  

Effects of amifostine and fengling polysaccharide on proliferation of peripheral blood hematopoietic stem/progenitor cells

作　　者：李翠萍[1] 陈宝安[2] 黄成垠[2] 周敏[2] 傅强[1] 陈津[1] 程欣[2] 高峰[2] 高冲[2] 丁家华[2] 孙耕云[2] 王俊[2] 程坚[2] 

机构地区：[1]南京红十字血液中心,210008 [2]东南大学附属中大医院血液科

出　　处：《江苏医药》2004年第4期254-256,共3页Jiangsu Medical Journal

基　　金：国家教委留学基金资助项目 ( 6890 0 0 10 0 2 ) ;南京市留学基金资助项目 ( 7690 0 0 40 2 6);南京市医学科技发展项目(YKK0 2 5 2 )

摘　　要：目的　研究氨磷汀 (WR 2 72 1)和峰龄多糖 (FLPS)对人外周血造血干 /祖细胞的刺激增殖及协同作用。方法　分离单个核细胞 (MNC) ,进行甲基纤维素半固体培养 ,观察氨磷汀和峰龄多糖对骨髓粒 巨噬细胞集落 (CFU GM)的作用。结果　经 0 0 1～ 5 0mmol/L氨磷汀 37℃作用 30分钟的MNC以及峰龄多糖 0 5～ 2 5 μg/ml时CFU GM集落数均显著高于对照组 (P <0 0 5 ) ,且集落较大。CFU GM平均集落数在对照组、WR 2 72 1(1mmol/L)组、FLPS(5 μg/ml)组和AMF +FLPS组每 10 5MNC分别为 91 4± 5 0 4、119 8± 6 2 9、14 3 2± 76 4和 179 2± 97 6 ,各组与对照组相比 ,差异有显著性 (P <0 0 5 ) ;AMF +FLPS组CFU GM集落数显著高于AMF和FLPS组 (P <0 0 1和 <0 0 5 )。结论　氨磷汀和峰龄多糖对CFUObjective To observe the effects of amifostine(WR-2721)and Fengling polysaccharide (FLPS)on proliferation of peripheral blood hematopoietic stem/progenitor cells.Methods Mononuclear cells were separated by Ficoll(1 077 g/ml)and cultured in methylcellulose semisolid medium.The effects of WR-2721 and FLPS on colony forming unit -granulocyte macrophage(CFU-GM)were studied.Results The number of CFU-GM was significantly increased in 10 samples by addition of 0 5-25 μg/ml FLPS and 12 samples treated with 0 01-5 mmol/L WR-2721 30 min at 37℃,P<0 05.The mean value of CFU-GM in groups of negative control,WR-2721(1 mmol/L),FLPS(5 μg/ml)and WR-2721+FLPS were 91 4±50 4?119 8±62 9?143 2±76 4 and 179 2±97 6 per 1×10 5 MNC,respectively.Compared with control,singnificant differences were seen between each group,P<0 05.WR-2721+FLPS group compared with WR-2721 and FLPS group,P<0 01 and P<0 05.Conclusion WR-2721 and FLPS can enhance the proliferation of hematopoietic stem/progenitor cells.Combination of WR-2721 and FLPS show stronger effect than the WR-2721 or FLPS alone.

关 键 词：氨磷汀 峰龄多糖 外周血 造血干细胞 细胞增殖 

分 类 号：R965[医药卫生—药理学] R331.2[医药卫生—药学]