大鼠三叉神经本体觉中枢通路上第三级核团内PV样阳性终末与丘脑投射神经元的突触联系(英文)  

THE SYNAPTIC CONNECTIONS BETWEEN PV-LI TERMINALS AND THALAMIC PROJECTION NEURONS IN THE THIRD-ORDER NUCLEI OF CENTRAL PATHWAY OF TRIGEMINAL PROPRIOCEPTIVE SENSATION OF THE RAT

作　　者：董玉琳[1] 李金莲[1] 

机构地区：[1]第四军医大学解剖学教研室,梁銶琚脑研究中心,西安710032

出　　处：《解剖学报》2004年第1期13-17,共5页Acta Anatomica Sinica

基　　金：NationalnaturalsciencefoundationofChina( 3 9870 2 62 )

摘　　要：目的　观察大鼠三叉神经本体觉中枢通路上第三级核团内Parvalbumin样阳性轴突终末与丘脑投射神经元之间是否存在突触联系。　方法　用HRP逆行追踪和包埋前免疫电镜相结合的双重标记法。将WGA HRP注入丘脑腹后内侧核逆行标记投射神经元。　结果　WGA HRP注入丘脑腹后内侧核 (VPM)后 ,WGA HRP标记神经元主要分布在感觉主核背内侧部 (Vpdm)、三叉上核尾外侧部 (Vsup CL)以及三叉神经运动核腹侧区 (AVM)和上橄榄核背侧区 (ADO)。电镜下可见PV样阳性神经元的轴突终末与WGA HRP标记的胞体或者树突形成突触联系。另外PV阴性神经元的轴突终末也与WGA HRP标记的胞体或树突形成突触联系 ,这些胞体或树突偶尔为PV阳性。　结论　在三叉神经本体感觉信息从第三级神经元向丘脑腹后内侧核 (VPM)传递的过程中 ,PV样阳性神经元可能通过突触传递机制而发挥作用。Objective To examine if axonal varicosities with parvalbumin-like immunoreactivity(PV-LI) might make synaptic connections with thalamic projection neurons in the third-order nuclei of central pathway of trigeminal proprioceptive sensation of the rat. Methods The HRP-retrograde tracing method combined with immuno-electron microscopy was used.Projection neurons were retrogradely labeled with wheat germ agglutinin-horseradish peroxidase(WGA-HRP) which was injected into the ventral posteromedial nucleus(VPM) of the thalamus. Results After injection,a number of WGA-HRP-labeled neurons were observed mainly in the principal sensory trigeminal nucleus(Vp),the caudolateral part of supratrigeminal nucleus (Vsup-CL),the area ventral to the motor trigeminal nucleus(AVM) and the area dorsal to the superior olivary nucleus(ADO).Electron microscopy confirmed that axon terminals with PV-LI made synaptic contact on somatic and dendritic profiles which were labeled with WGA-HRP.In addition,some PV-negative axon terminals made synapses with WGA-HRP-labeled somatic or dendritic profiles occasionally showed PV-LI.Conclusion It was indicated that some of PV-containing projection neurons might be involved in the transmission of the trigeminal proprioceptive information from the third-order nuclei to the VPM of thalamus through the mechanism of synaptic transmission.

关 键 词：大鼠 三叉神经本体觉 中枢通路 PV样阳性终末 丘脑投射神经元 突触 电镜免疫组织化学 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学]