神经生长因子诱导神经干细胞定向迁移  

NERVE GROWTH FACTOR(NGF) INDUCES DIRECTIONALMIGRATION OF NEURAL STEM CELLS

作　　者：施英唐[1] 马骥[1] 袁崇刚[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062

出　　处：《解剖学报》2004年第1期22-25,共4页Acta Anatomica Sinica

基　　金：教育部高等院校骨干教师资助项目

摘　　要：目的　探讨体外培养环境中神经生长因子 (NGF)诱导神经干细胞定向迁移的特性。　方法　运用半固体培养法结合单细胞克隆技术动态观察神经干细胞迁移情况 ,采用免疫细胞化学技术鉴定迁移细胞的类别。　结果　在具有NGF浓度梯度的培养体系中 ,克隆细胞迁向高浓度NGF区域。在NGF源附近 ,这种定向迁移更加明显。迁移细胞随时间的延长表达神经元特异性抗原。在含均匀浓度NGF的培养基中细胞从球团向四周短距离的扩散 ,无方向性。所有迁出细胞随时间的延长进一步分化成熟 ,干细胞比率下降。　结论　NGF有诱导神经干细胞定向迁移的作用。Objective To investigate the effect of nerve growth factor(NGF) on inducing directional migration of neural stem cells in vitro. Methods Semi-solid cell culture method was used to observe the cell′s directional migration;immunocytochemical method was used to analyse the neuronal phenotype. Results In the semi-solid medium with gradient concentration of NGF,neural stem cells migrated directionally from lower concentration of NGF to higher concentration,and the nearer to the source of NGF,the more evident the result.The migrated cells show neuron charcteristics.In the medium with NGF but with no gradient concentration,the cells migrated without direction.Conclusion NGF could induce directional migration of neural stem cells.

关 键 词：神经生长因子 诱导 神经干细胞定向迁移 半固体培养 大鼠 

分 类 号：Q256[生物学—细胞生物学] Q421