主动脉平滑肌细胞在三维培养中产生弹性纤维的研究  

AORTIC SMOOTH MUSCLE CELLS PRODUCED ELASTIC FIBERS IN THREE-DIMENSIONAL CULTURE

作　　者：余灵祥[1] 邹仲之[1] 

机构地区：[1]第一军医大学组织学胚胎学教研室,广州510515

出　　处：《解剖学报》2004年第1期74-78,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金项目 ( 3 9870 810 );广东省自然科学基金项目 ( 970 3 41)

摘　　要：目的　研究主动脉平滑肌细胞 (ASMC)在仿真皮替代物的三维培养中是否能产生弹性纤维。　方法　取 1周龄SD大鼠胸主动脉 ,进行原代和传代培养 ,以α肌动蛋白免疫细胞化学染色鉴定平滑肌细胞。然后 ,将ASMC与胶原、甲壳素及糖胺多糖组合形成的凝胶混合 ,进行三维培养 ;培养 1周和 2周后 ,用Gomori醛复红染色和弹性蛋白免疫细胞化学染色 ,检测ASMC胶原凝胶。　结果　传代细胞经α肌动蛋白免疫细胞化学染色鉴定 ,98%以上为ASMC。培养 1周和 2周的ASMC胶原凝胶中 ,均显示有弹性纤维存在。　结论　ASMC在仿真皮替代物的三维培养中能够产生弹性蛋白 ,并形成弹性纤维。Objective To Study if aortic smooth muscle cells(ASMC) can produce elastic fibers in three-dimensional culture imitating the dermis substitute. Methods Primary culture of ASMCs were obtained by explanting of thoracic aortae from one-week old SD rats. α-actin immunocytochemistry was used to approve the smooth muscle cells. After subculture, ASMCs were mixed with gels composed of collagen, chitin and glucosaminoglycans, then, three-dimensional culture was carried out. After 1 week and 2 weeks, Gomori aldehyde fuchsin staining and elastin immunocytochemistry were applied to detect the elastic fibers in the ASMC-collagen gels. Results More than 98% of the cultured cells were approved as smooth muscle cells by α-actin immunocytochemistry. After both 1 and 2 weeks of three-dimentional culture, elastic fibers were detected in the ASMC-collagen gels by both stainings.Conclusion ASMCs can synthesize elastin and produce elastic fiber in three-dimentional culture imitating the dermis substitute.

关 键 词：主动脉平滑肌细胞 三维培养 弹性纤维 真皮替代物 大鼠 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]