三维培养的主动脉平滑肌细胞-胶原凝胶皮下移植的研究  

THE STUDY ON TRANSPLANTATION OF THREE-DIMENTIONAL CULTURED ASMC-COLLAGEN GEL TO SUBCUTANEOUS

作　　者：余灵祥[1] 邹仲之[1] 

机构地区：[1]第一军医大学组织学胚胎学教研室,广州510515

出　　处：《解剖学报》2004年第1期79-82,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金项目 ( 3 9870 810 );广东省自然科学基金项目 ( 970 3 41)

摘　　要：目的　研究经三维培养后的主动脉平滑肌细胞 (ASMC) 胶原凝胶移植后 ,是否仍能产生弹性纤维及宿主的接受状态。　方法　将仿真皮替代物三维培养 2周的ASMC 胶原凝胶植入大鼠皮下 ,分别于术后 4、7、10、14和 2 8d取材 ,用HE染色、Gomori醛复红染色、α肌动蛋白免疫组织化学染色和弹性蛋白免疫组织化学染色 ,观察和检测ASMC 胶原凝胶。　结果　在移植后第 1周有大量的弹性纤维产生 ,但于 2 8d时完全消失。在移植后的 2 8d内 ,ASMC 胶原凝胶块周围没有明显的白细胞浸润。　结论　利用ASMC 胶原凝胶作为一种有弹性的真皮替代物 ,尚需进一步的研究。Objective To investigate if aortic smooth muscle cells (ASMCs) cultured three-dimentionally with collagen gel can still produce elastic fibers and the acceptance of hosts after transplantated in subcutaneous of rats. Methods AMSC-collagen gels that had been cultured for 2 weeks by three-dimentionally imitating the dermis substitute were transplanted to rats subscutaneous. After 4,7,10,14 and 28 days, the transplantations were examined via HE staining, Gomori aldehyde fuchsin staining, α-actin immunohistochemistry and elastin immunohistochemistry. Results The amount of elastic fibers increased significantly in the week, whereas on the 28th day, no elastic fibers were detected.There was no remarkable leucocyte infiltration around the ASMC-collagen gel during 28 days.Conclusion Further studies are needed to explore the ASMC-collagen gels as a kind of elastic dermis substitute.

关 键 词：三维培养 主动脉平滑肌细胞 胶原凝胶 皮下移植 

分 类 号：R329.3[医药卫生—人体解剖和组织胚胎学]