Assessment of Genetic Variation and Distribution Pattern of Thalictrum petaloideum Detected by RAPDs  

作　　者：XIELei LILiang—Qian ZHANGDa—Ming 

机构地区：[1]LaboratoryofSystemaricBeijing100093,China

出　　处：《Acta Botanica Sinica》2004年第2期165-170,共6页Acta Botanica Sinica（植物学报：英文版）

摘　　要：Random amplified polymerphic DNA(RAPD)method was applied to assessg enetic variation and population structure of Thahctrum petalotdeum L(Ranunoulaceae)，Two hundred and forty-six individuals from 11 populations of the species were investigated by RAPD profiles Twenty selected RAPD primers generated 125 bands．in which 120 were polymorphic Ther esults revealed a high level of genetic variation(ercentage of polymorphIc bands(PPB was 96％．Nei’s gene diversity(りwas 03502 and shannon’s information index(I) was 0．5199 at the species level) The differentiation among the populations was high(Gst=0．3511)in this species．Result of analyzing of molecularvariance(AMOVA)showedthat38．88％of genetic variance was found among the populations Positive correlation withr r=01945(P=00002)was found between genetic distance and geographic distance amongpo pulations Two populations distributed in the drainage basin of YanELz River affined genedcally and formed one clada and the rest nine populations formed the other clade in both unweighted pair-group method using arithmetic average(UPGMA)trees made by two different method different methods. It was yen/clear that these two populations were very special, andmust be closely related in history, despite the fact that they now share quite weak link to the restpopulations through gene communication.

关 键 词：瓣蕊唐松草 随机扩增多态DNA RAPD 遗传多样性 地理分布 毛茛科 

分 类 号：Q949.746.5[生物学—植物学] Q948