^(60)Coγ射线对高免卵黄液中EDS-76病毒灭活的研究  

Effect of ^(60) Coγ Rays Inactivation on the EDS-76 Virus in High Immunity Egg Yolk

作　　者：古少鹏[1] 郑明学[1] 韩克光[1] 柴桂珍 马海利[1] 

机构地区：[1]山西农业大学动物科技学院,中国山西太谷030801 [2]山西省兽药监察所,中国山西太原030000

出　　处：《激光生物学报》2004年第2期111-115,共5页Acta Laser Biology Sinica

基　　金：山西省科委攻关项目 ( 9710 15)

摘　　要：用不同剂量、剂量率的6 0 Coγ射线对卵黄液中的EDS 76病毒进行辐照 ,研究了其对病毒的灭活效果和对卵黄抗体 (IgY)的影响。结果表明 ,6 0 Coγ射线辐照 ,可以灭活高免卵黄液中的EDS 76病毒 ,且随辐照剂量、剂量率的增大 ,灭活率也增高 ;EDS 76病毒在卵黄液中的D10 值为 0 .57kGy～ 0 .60kGy ;6.0kGy的辐照剂量 ,可以将高免卵黄液中的EDS 76病毒完全灭活 ,且不影响卵黄抗体效价 ,该卵黄抗体稳定性好、耐酸、耐热。EDS 76 virus in the yolk were irradiated using different dosage, dosage rate of 60 Coγ,and the inactivation effect and the effect on yolk antibody (IgY)were studied. The results showed that EDS 76 virus could be killed and the inactivation rate increased with the increase of irradiation dosage and dosage rate . The D 10 value of EDS 76 virus in the yolk was 0.57～0.60kGy; EDS 76 virus could be totally inactivated by the dosage of 6.0kGy, and the antibody titre still didn't change and showed good resistance against heat, acid and trypsase.

关 键 词：高免卵黄液 IGY ^60Coγ射线 灭活 EDS-76病毒 

分 类 号：S852.65[农业科学—基础兽医学]