根瘤菌培养基的优化和剂型的比较研究  

MEDIUM OPTIMIZATION AND INOCULANT TYPE COMPARISON OF RHIZOBIUM

作　　者：吴红慧[1] 周俊初[1] 

机构地区：[1]华中农业大学农业微生物学国家重点实验室,武汉430070

出　　处：《微生物学通报》2004年第2期14-19,共6页Microbiology China

基　　金：欧盟国际合作计划课题 (No ICA4 CT 2 0 0 1 10 0 5 6 );国家 86 3计划课题 (No 2 0 0 1AA2 14 0 2 1)

摘　　要：以费氏中华根瘤菌 (Sinorhizobiumfredii)HN0 1和大豆慢生根瘤菌 (Bradyrhizobiumjaponi cum)USDA1 1 0作为供试菌 ,进行YMA、TY、SM、PA和BSE等 5种培养基的比较试验。结果表明 ,两种菌都在BSE中生长速度最快。对USDA1 1 0进行培养基的优化试验 ,筛选出一种能将生长速度提高 2倍左右的优化培养基。制作了供试菌的固体、液体与冻干菌剂 ,结果表明在 3种供试固体剂型载体中 ,草炭要优于蛭石 ,蛭石又优于珍珠岩。供试菌在两种液体剂型中的存活率和活菌数均较高 ,氮气或真空剂型对存活率的影响没有明显的差别。两种冻干菌剂的结果表明 ,供试菌在冻干过程中的死亡率较高 ,液氮冻干优于常规冻干。冻干菌剂在储存条件下的存活率为 : 2 0℃ >4℃ >BSE media was screened from five media including YMA, TY, SM, PA and BSE as the best for the growth of representative strain Sinorhizobium fredii HN01 and Bradyrhizobium japonicum USDA110. Based on YMA, one optimized medium was obtained from orthogonal tests. According to survival percentage, concentrated liquid type was proved to be the highest among solid, liquid and freeze-dried inoculant types tested. Peat was better than vermiculite and the latter was better than perlite among three solid inoculant carriers tested. Freeze-dry method was not suitable for the production of rhizobium inoculant since the high death ratio during freeze-dry process. The survival percentage of freeze at liquid nitrogen was higher than at -20℃ and the order for its storage was -20℃>4℃>room temperature.

关 键 词：大豆根瘤菌 培养基优化 剂型 

分 类 号：S144[农业科学—肥料学]