西北地区甘草根瘤菌的表型多样性研究  

PHENOTYPIC FEATURE DIVERSITIES OF RHIZOBIA ISOLATED FROM GLYCYRRHIZA LINN IN NORTHWEST ARID-SEMIARID REGION OF CHINA

作　　者：杨亚珍[1] 韦革宏[1] 万晓红[1] 陈春[1] 

机构地区：[1]西北农林科技大学资源环境学院,杨凌712100

出　　处：《微生物学通报》2004年第2期20-25,共6页Microbiology China

基　　金：国家自然科学基金资助项目 (No 30 0 0 0 0 0 5 );教育部全国优秀博士学位论文作者专项基金 (No 2 0 0 2 5 4 );国家 973课题 (G2 0 0 0 0 186 0 5 )~~

摘　　要：选用分离自西北干旱、半干旱地区的甘草根瘤菌 68株和 3 4株参比菌株 ,进行了 1 1 3项表型性状测定 ,结果表明 :不同地理来源、同一地理来源、甚至同一植株不同根瘤菌株在碳氮源利用、抗生素敏感性、抗逆性等方面存在着差异。部分菌株具有较强的耐盐耐碱能力 ,其中有 5株和 1株菌分别能耐受 5 0g L、 60g L的NaCl;有 43 %的菌株能在初始pH1 2的YMA培养基上生长。从数值分类树状图谱发现 ,在 85 5 %的相似水平上供试菌株构成了 3个表观群。群I有 2 7株菌 ,除 4株来自陕西外 ,其余均来自新疆。群II有 4株菌 ,皆来自陕西。群III有 8株菌 ,6株来自陕西 ,2株来自宁夏 ,它们与R hainanense聚在一起。而群I、群II没有与参比菌株聚在一起 ,可能是新的表观群 。phenotypic features of 68 strains isolated from Glycyrrhiza Linn, growing in northwest arid-semiarid region of China, were analyzed. 34 reference strains were included for comparison. The result showed that there were great diversities among strains isolated from different geographic origin, the same geographic origin, and the different root nodules of the same root in carbon and nitrogen nutrient utilization, resistance to antibiotics, endurance to salt, alkali. Some strains had the properties of endurance to salt and alkali. 5 strains and 1 strain could endure 50g/L, 60g/L NaCl respectively. 20% and 43% strains could grow in YMA medium with pH5, pH12. Numerical taxonomy showed that the strains formed 3 groups at 85.5% similarity level. Group I contained 27 strains, 23 strains isolated from Xinjiang, 4 isolated from shannxi. Group II had 4 strains isolated from shannxi. Group III ( 6 strains isolated from shannxi, 2 isolated from Ningxia) were clustered into R.hainanense. Group I and group II may be new groups, which were not clustered into the reference strains, and there taxonomic status must be confirmed by further measurements.

关 键 词：甘草 根瘤菌 表型多样性 

分 类 号：S154[农业科学—土壤学]