α-半乳糖苷酶酶解B型长臂猿细胞回输A型长臂猿的研究  

B Gibbon Red Blood Cells Digested by α-Galactosidase Transfused to A Gibbon

作　　者：刘泽澎[1] 张成林[2] 卢雁平[2] 杨明海[2] 张金国[2] 高新[1] 杨军[1] 贾延军[1] 高红伟[1] 任会明[1] 李素波[1] 章扬培[1] 

机构地区：[1]军事医学科学院输血研究所,北京100850 [2]北京动物园,北京100044

出　　处：《中国生物化学与分子生物学报》2004年第2期229-233,共5页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家 973项目 (No .2 0 0 2CB713 80 4);全军十五重点课题 (No .2 0 0 0 2 5 2 910 )~~

摘　　要：使用基因重组咖啡豆α 半乳糖苷酶体外处理B型长臂猿红细胞 ,使其转变为O型 ,再回输给A型长臂猿 .α 半乳糖苷酶可以清除B型长臂猿红细胞表面B抗原 ,而不影响红细胞结构、功能及其在受体体内存活 .α 半乳糖苷酶酶解的B型红细胞输给A型血的长臂猿 ,未发生输血反应 ,受血猿的血液及尿液常规指标与输血前相比 ,无明显变化 .In order to perform the animal transfusion experiment before entering clinical trial of B→O blood conversion,B type of gibbon red blood cells were digested by recombinant coffee bean α galactosidase which resulted in B→O blood conversion.The converted red blood cells were transfused into the recipient bibbon with A blood type.The results showed that α\|galactosidase could remove blood B antigen located on the surface of gibbon red blood cells efficiently.There were no harmful effects on donor red blood cell structure and function as well as on donor cell survival in recipient gibbon after α\|galactosidase digestion.The results also demonstrated that no transfusion rejection happened in recipient gibbon with different blood group.The routine blood and urine tests of recipient gibbon were normal after enzymatically converted blood cell transfusion.

关 键 词：Α-半乳糖苷酶 红细胞B抗原 长臂猿 输血 血液 

分 类 号：R457.11[医药卫生—治疗学]