牛-兔种间重组胚体外发育能力的研究  

Study on Development of Cloned Embryo Using Bovine Somatic Cell and Rabbit Oocyte in vitro

作　　者：刘亚[1] 章孝荣[1] 陈大元[2] 张运海[3] 章志国[1] 金仁桃[1] 汪存利[1] 章美玲[1] 李东伟[1] 李斌[1] 赵焕[1] 程立子[1] 

机构地区：[1]安徽农业大学畜牧水产学院,合肥230036 [2]中国科学院动物研究所生殖生物学国家重点实验室,北京100080 [3]中国农业大学生物学院,北京100094

出　　处：《中国农业科学》2004年第3期441-445,共5页Scientia Agricultura Sinica

基　　金：安徽省"十五"重大科技专项资助项目(01603006)

摘　　要：以超排的青年母兔和经产母兔卵母细胞为受体细胞,以处于休眠期和非休眠期的黑毛和牛耳成纤维细胞为核供体,共获得180枚种间重组胚,采用M199+10%FBS和RD+10%FBS2种培养液进行体外培养,有112枚发育到2-细胞期(62.2%),26枚发育到桑椹胚期(14.4%),20枚发育到囊胚期(11.1%)。结果显示,供体细胞处于休眠期与否,对重组胚的卵裂率无显著影响(P<0.05),但以休眠期细胞为核供体的重组胚8-细胞～16-细胞胚的发育率及桑椹胚发育率显著高于非休眠期细胞的重组胚(P<0.05),二者在囊胚发育率上差异极显著(P<0.01);以M199为培养液的胚胎在卵裂率、桑椹胚发育率和囊胚发育率上与RD组无显著差异(P>0.05)。Total 180 reconstituted embryos were produced by using Japan Black Cattle's ear fibroblast cell whichstaying at G0 or non-G0 cell cycle stage as donor and oocyte superovulated from multiparous or young rabbit as recipient.After cultured in two kinds of medium: M199+10%FBS or RD+10%FBS, 112 of them developed to 2-cell stage (62.2%), 26developed to morula stage (14.4%), 20 developed to blastocyst stage (11.1%). The result indicated that no significantdifference was obsearved in cleavage rate between two groups of reconstituted embryo which using G0-stage cell and non-G0 stage cell as donor respectively, but when used G0-stage cell as donor, 8-cell-16-cell rate and morula rate were raisedsignificantly (P<0.05), blastocyst rate was raised significantly (P<0.01). No significant difference in cleavage rate, morularate and blastocyst rate was obsearved when using two kinds of culture medium (P>0.05).

关 键 词：牛 兔 种间重组胚 体外发育能力 种间核移植 卵裂率 囊胚发育率 桑椹胚发育率 

分 类 号：S813[农业科学—畜牧学]