水稻长穗颈基因eui2(t)共分离SSR标记的获得  

Identification of a SSR Marker Co-segregating with the Rice Elongated Uppermost Internode Gene eui2(t)

作　　者：朱宏波[1] 方宣钧[2] 杨仁崔[1] 

机构地区：[1]福建农林大学作物遗传育种研究所,福州350002 [2]海南省热带农业资源开发利用研究所,三亚572025

出　　处：《中国农业科学》2004年第3期456-459,共4页Scientia Agricultura Sinica

基　　金：福建省自然科学基金重大资助项目(2001F002)

摘　　要：水稻长穗颈突变体(eui2)是通过人工诱变获得的,突变基因已经被初步定位在水稻第10染色体的长臂中部。本研究以含有eui2(t)基因的籼稻品系协青早eB2与粳稻品种中花14杂交的F2代(8000个单株)作为定位群体,借助已发表的水稻SSR标记及根据GenBank中的水稻基因组序列设计的SSR标记,找到1个与eui2(t)基因共分离的标记RZH38,为eui2(t)基因的辅助育种提供了分子标记。A rice elongated uppermost internode (eui2) mutant was obtained by gamma ray irradiation, of which themuntant gene had been mapped on the middle of the long arm of chromosome 10. An F2 population including 8000individuals developed from a cross between the eui2(t) mutant Xieqingzao eB2 (Indica) and Zhonghua 14 (Japonica), wasused to search for new markers more tightly linked to eui2(t). Using the published SSR markers was found and the new SSRmarkers designed according to the public genomic DNA sequences, found a SSR marker RZH38 co-segregating with thegene eui2 (t). This marker could facilitate marker-assisted selection of eui2(t) gene.

关 键 词：水稻 长穗颈基因 eui2(t)基因 共分离 SSR标记 分子标记辅助育种 

分 类 号：S511[农业科学—作物学]