15-羟廿碳四烯酸对大鼠缺氧性肺动脉环的作用  

Effect of 15-HETE on the hypoxic pulmonary arterial rings

作　　者：孟丽巍[1] 龚秀萍[1] 姚红娟[1] 王静[1] 朱大岭[2] 

机构地区：[1]哈尔滨医科大学第一临床医学院麻醉科,150001 [2]黑龙江省生物医药工程重点实验室-省部共建国家重点实验培育基地

出　　处：《中华麻醉学杂志》2004年第1期25-28,共4页Chinese Journal of Anesthesiology

摘　　要：目的 研究15-羟廿碳四烯酸(15-HETE)对缺氧(FiO2 10％)大鼠第三级肺动脉环的作用,以探讨15-HETE在大鼠缺氧性肺血管收缩(HPV)中的作用,为肺动脉高压及其并发症的手术麻醉采取相应的治疗措施,以减少低氧血症的发生。方法 16只健康Wistar大鼠随机分为两组(n=8):A组为对照组,吸大气,其吸入氧浓度(FiO2)为21％;B组为缺氧组,即将大鼠置于FiO2为10％的缺氧箱中。9d后将大鼠处死,游离直径为0.5-1.0 mm肺动脉(PA)剪成3mm长的血管环,血管环在37℃的KH液中平衡40min。KH液中15-HETE的浓度逐渐从10-8增至10-6mol·L-1,分别加入不同K+通道阻断剂后,再加入15-HETE。肺动脉环收缩张力信号通过Medlab-U-4CS生物信号采集处理系统的四通道接头传输到计算机相应处理软件上进行数据记录和分析,并根据血管张力的变化绘制浓度反应曲线;分别计算出收缩率。结果 15-HETE以浓度依赖方式(10-8-10-6mol·L-1)使游离肺动脉环张力明显增加(P<0.05),其在B组中作用更为明显。2mmol·L-1 4-AP、2mmol·L-1 4-AP+10-6mol·L-115-HETE使肺动脉环张力显著性增高(P<0.05),二组间比较,差异无显著性(P>0.05);10-2mol·L-1TEA、10-6mol·L-1GLYB,对血管环张力无明显影响(P>0.05);10-2mol·L-1 TEA+10-6mol·L-115-HETE和10-6mol·L-1Objective To inveatigate the effect of 15-HETE, a metabolite of arachidonic acid, on isolated hypoxic pulmonary arterial ringa ( PARs) , trying to find appropriate treatment for pulmonary hypertension and its complications during anesthesia in order to avoid hypoxemia. Methods Sixteen healthy Wistar rats of either sex weighing (230 ± 10) g were randomly divided into two groups : A control group breathing fresh air (FiO2 =21%) and B hypoxia group breathing hypoxic air (N2 = 90% , O2 = 10% ) in a hypoxic box. After breathing hypoxic air for 9 days the animals were anesthetized. Heart and lungs were immediately removed and PARs (0.5-1.0 mm in diameter and 3 mm in length) were prepared. Four PARs were prepared from each animal. The PARs were suspended in baths filled with Krebs-Hensleit (K-H) solution maintained at 37℃ and aerated with 95% O2 and 5% CO2. Preload was gradually increased to 0.3 g in 30 min. The isometric tension was measured using a four-channel force-displacement transducer. 15-HETE was added to K-H solution and the concentration was gradually increased from 10-8 to 10-6 mol·L-1 at 5 min intervals. Contractility of PARs was analyzed by a software of Medlab 6.0. Concentration-tension curve was drawn and contraction rates were calculated. 2 mmol·L-1 4-AP, 10-2 mol·L-1 TEA and 10-6 mol·L-1 GLYB were added to separate K-H solution baths and 40 min later 15-HETE was added in order to detennine the effect of difierent potassium channel blockers on contraction response of PARs to 15-HETE. Results With increasing concentration from 10-8 to 10-6 mol·L-1 , 15-HETE increased PARs tension gradually in a dose-dependent manner from 106% ±6% to 139% ± 4% in group A and from 113% ±6% to 163% ±6% in group B. The difference in PARs tension between group A and B was statistically significant (P<0.05). 2mmol·L-1 4-AP increased PARs tension to 135%±4% in group A and to 134%±7% in group B but addition of 10-6 mol·L-1 15-HETE did not further increase PARs tension in both groups. 10-2 mol ·L-1 TEA and 10-6 m

关 键 词：15-羟廿碳四烯酸 大鼠 肺动脉环 肺动脉高压 低氧血症 缺氧性肺血管收缩 麻醉 

分 类 号：R96[医药卫生—药理学]