背根神经节压迫对大鼠痛行为及脊髓背角内降钙素基因相关肽的影响  

Changes in behavior and spinal cord dorsal horn CGRP following chronic compression of DRG in rats

作　　者：宋雪松[1] 高金贵[2] 孙木[3] 杨桐伟[1] 王凡[1] 

机构地区：[1]吉林大学第一医院麻醉科,长春130021 [2]河北医科大学第二医院麻醉科 [3]吉林大学第一医院基础医学院生理教研室,长春130021

出　　处：《中华麻醉学杂志》2004年第1期44-47,共4页Chinese Journal of Anesthesiology

摘　　要：目的 建立有效的背根神经节慢性压迫模型,探讨大鼠痛行为及脊髓背角内降钙素基因相关肽的变化。方法 采用铬制1-0肠线通过椎间孔压迫大鼠L4、L5背根神经节制备压迫模型,术后观察缩爪阈值变化,用放射免疫分析方法测定脊髓背角中降钙素基因相关肽的含量。结果 压迫组术后痛敏明显,术后1周时达高峰,以后逐渐恢复,但到术后4周时仍未完全达到正常水平;假手术组术后痛敏恢复较快,于术后7d恢复至正常水平。压迫组背角内降钙素基因相关肽含量在术后5d及4周时均明显增高,与同组对侧、假手术组及对照组相比差异有显著性。光镜观察,压迫组术后5d压迫侧背根神经节出现明显的神经内膜水肿及神经元胞质水肿,到术后4周这种改变虽然仍存在,但明显减轻。结论 稳定可靠的背根神经节慢性压迫模型,受压侧脊髓背角中降钙素基因相关肽含量持续升高,与慢性痛行为一致。Objective To develop a reliable and reproducible modei of hyperalgesia arising from chronic compression of dorsal root ganglion (DRG) and investigate the contribution of spinal cord dorsal horn calcitonin gene-related peptide (CGRP) to the clinical manifestation of sciatica.Methods Sixty healthy Wistar rats of either sex weighing 200-300 g were randomly divided into three groups: group A control ( n = 12) ; group B sham operation (n = 24) and group C chronic compression of DRG ( n = 24) . In group B and C the animals were anesthetized with intraperitoneal 1.25% thiopental sodium (50mg·kg-1) . The animals were then placed in prone position and the left L4 and L5 intervertebral foramina were exposed. In group C a 4 mm long 1-0 chromic catgut was inserted into the intervertebral foramen. Motor function of the hind limbs was assessed by gait disturbance scores (GDS) , and paw-withdrawal threshold (PWT) was measured with modified Randall algometer 3, 5, 7, 10, 14, 17, 21 and 28 days after operation. In group B and C 12 animals were decapitated on the 5th and 28th days after operation respectively and lumbar section of spinal cord was removed for determination of dorsal horn CGRP content (by radio-immunoassay) and microscopic examination of DRG. Results In chronic compression group (C) significant hyperalgesia occurred after operation and reached a peak one week after operation then gradually decreased but didn't return to normai on the 28th day, while in sham operation group (B) postoperative hyperalgesia quickly decreased and returned to normai on the 7th day. In group C dorsal horn CGRP content on the compressed side significantly increased on the 5th and 28th day after operation as compared with that on the contralateral side in the sham operation and control group. Microscopic examination of DRG showed significant edema of endoneurium and neurons which persisted on the 28th day but significantly attenuated. Conclusion This animal model reliably produces a disorder resembling low back pain. It is easy to perform l

关 键 词：背根神经节 大鼠 疼痛 脊髓 降钙素基因相关肽 神经压迫综合征 放射免疫法 动物模型 

分 类 号：R681.5[医药卫生—骨科学]