Hsp70/CD80嵌合DNA疫苗对结核杆菌的治疗作用  

Study on the role of the chimeric hsp70/CD80 DNA vaccine for treating infection of Mycobacterium tuberculosis

作　　者：史小玲[1] 李晖[1] 钟森[1] 张建军[1] 邓存良[1] 黄永茂[1] 卢润生[2] 吴建林[2] 卫帮富[2] 蒋绍双[2] 

机构地区：[1]四川省泸州医学院附属医院传染与免疫实验室,646000 [2]四川省结核病防治研究所

出　　处：《中华传染病杂志》2004年第1期30-33,共4页Chinese Journal of Infectious Diseases

基　　金：国家自然科学基金 ( 3 9870 70 3 )

摘　　要：目的　研究结核病Hsp70 /CD80嵌合DNA疫苗结核杆菌感染小鼠的治疗作用。 方法用结核杆菌强毒株攻击小鼠后 ,接种Hsp70 /CD80嵌合DNA疫苗 ,观察该疫苗对结核杆菌感染的治疗作用。结果　疫苗接种后 14d ,测得卡介苗组、注射 pcDNA3组、注射 pcDNA3 hsp70组及Hsp70 /CD80嵌合DNA疫苗免疫小鼠的血清IFN γ分别为 (12 1.5 4 +5 6 .39) pg/ml、(192 .0 0 +6 4 .36 )pg/ml、(5 4 2 .33+99.77) pg/ml及 (1336 .98+12 9.6 4 ) pg/ml,嵌合DNA疫苗组与前三组比较差异有显著性 (P <0 .0 1)。肝、脾组织涂片细菌计数及组织培育菌落记数 ,嵌合DNA疫苗组与其他组比较差异有显著性 (P <0 .0 1)。结论　Hsp70 /CD80嵌合DNA疫苗与卡介苗及单一hsp70DNA疫苗相比 ,具有更好的免疫治疗效果。Objective To study the role of the chimeric hsp70/CD80 DNA vaccine for treatment for TB. Methods C57BL/6N mice challenged with H37Rv were immunized with the chimeric hsp70/CD80 DNA vaccine, hsp70 DNA vaccine and BCG in order to compare the therapeutic role of these vaccines. Results The levels of interferon γ (IFN-γ) in the serum of mice in hsp70/CD80 group (1336.98±129.64) pg/ml was significantly higher than in group BCG (121.54±56.39) pg/ml, pcDNA3 (192.00±64.36) pg/ml and pcDNA3 hsp70 (542.33±99.77) pg/ml. The growth of Mycobacterium tuberculosis in live and spleen were inhibited significantly in hsp70/CD80 vaccinated mice. Conclusions The chimeric hsp70/CD80 DNA vaccine was more efficient than BCG and hsp70 DNA vaccine alone in treating TB.

关 键 词：结核 DNA疫苗 CD80抗原 热休克蛋白质类70 治疗作用 

分 类 号：R392[医药卫生—免疫学]