^(125)I-抗MIF McAb体内检测同种移植排斥过程中MIF的产生和分布  

Biodistribution of ^(125)I labeled anti-MIF McAb in the skin allograft rejection of mice

作　　者：侯桂华[1] 韩建奎[2] 于敏[2] 刘德宜[1] 张超[1] 

机构地区：[1]山东大学医学院实验核医学研究所,济南250012 [2]山东大学齐鲁医院核医学科

出　　处：《中华核医学杂志》2004年第1期49-51,i003,共4页Chinese Journal of Nuclear Medicine

摘　　要：目的　探讨12 5I标记抗巨噬细胞移动抑制因子 (MIF)单克隆抗体 (McAb)体内检测小鼠同种皮肤移植排斥过程中MIF的产生和分布情况。方法　①利用Iodogen法 ,用Na12 5I标记抗MIFMcAb(Ⅲ D 9)及对照抗体 (HB4 5 )。②制备小鼠同种皮肤移植模型 ,并作组织学鉴定。③分别腹腔注射12 5I Ⅲ D 9和12 5I HB4 5 ,观察同种移植排斥过程中MIF在小鼠体内的产生和分布。结果　①12 5I Ⅲ D 9标记率为 76 6 7% ,比活度为 2 9 5 2TBq/mmol。②小鼠同种移植皮片的排斥期平均为 (13 4±2 5 )d。病理切片示 ,在排斥高峰期细胞浸润明显增多 ,主要为巨噬细胞和淋巴细胞。③移植术后早期 ,12 5I Ⅲ D 9和12 5I HB4 5在移植皮片中的放射性均高于正常皮肤 ,但两者无明显差异。在移植排斥期 (移植后 12d) ,12 5I Ⅲ D 9在移植皮片中的放射性明显高于12 5I HB4 5组 (P <0 0 1)。结论　小鼠同种皮肤移植排斥反应过程中MIF表达量随排斥反应出现明显增加 ,并在移植皮片局部呈特异性分布 ,提示MIF表达量增高与移植排斥反应密切相关。Objective To investigate the production and biodistribution of macrophage migration inhibition factor (MIF) during allograft rejection using 125 I labeled anti-MIF monoclonal antibody (McAb). Methods ①Anti-MIF McAb (Ⅲ.D.9) was radio-iodinated (Na 125 I) by Iodogen. ②Skin allografting mice model was established and the rejection was observed and confirmed daily histologically. ③The radioactivities in various normal tissues and in the skin allografts of model mice at different intervals after transplantation were measured. Results ①The allograft rejection period lasted (13.4±2.5) d with a dense macrophage and lymphocyte infiltration. ②Transplanted mice received injection of 125 I labeled anti-MIF McAb the high levels of radioactivities were found from day 1 and 3,then decreased,but it increased greatly from day 10 till day 14,then decreased again;and the transplanted mice received injection of 125 I labeled control antibody showed increased radioactivities from day 1 to day 3 and a slight increase during day 10 to 14. No obvious difference in other organs were found between 125 I labeled anti-MIF Abs and control Abs injection groups. Conclusions MIF expression during allograft rejection process shows a highly significant correlation with macrophage and lymphocyte accumulation in the skin grafts. The up-regulation of MIF expression during rejection provides a possible method for radioimmunoimaging of graft rejection.

关 键 词：巨噬细胞游走抑制因子 抗体 单克隆 同种移植 移植物排斥 

分 类 号：R817.4[医药卫生—影像医学与核医学]