小细胞肺癌单克隆抗体2F7F_((ab′)2)片段的^(99)Tc^m标记试剂盒的制备  

A study of ^(99)Tc^m labelling of monoclonal antibody 2F7F_((ab′)2) segment against small cell lung cancer

作　　者：黄飚[1,2] 张祥瑞[1] 朱岚[1] 莫忠根[3] 杨瑶琴[3] 赵新泰[3] 

机构地区：[1]江苏省原子医学研究所 [2]江南大学生物工程学院无锡214036 [3]上海市肿瘤研究所

出　　处：《核技术》2004年第5期354-356,共3页Nuclear Techniques

基　　金：国家 863 计划资助(2001AAA215141)申请国家一类新药(西药申临字第 97190 号)

摘　　要：采用2-亚氨噻酚盐酸盐修饰2F7F(ab′)2,通过葡庚糖酸转换,将Tcm标记在小细胞肺癌单克隆抗体2F799F(ab′)2上,并测定抗体的修饰率、标记率、胶体含量、免疫活性、稳定性及安全性,探讨一种高效便捷的Tcm-2F799F(ab′)2放免显像药盒的研制方法。结果显示:已修饰2F7F(ab′)2的修饰效率(巯基/分子)为6.3,99Tcm的标记率为93.5%,99Tcm胶体含量为2.5%,修饰抗体有良好的稳定性和免疫活性;;进行无菌、无热原、急慢性毒性实验,结果表明各项指标符合中国药典规定,所以建立的高效、便捷的单抗片断放免显像方法可以适应药盒化发展方向,该修饰、标记技术将对单抗F(ab′)2的放免显像的应用起到良好的作用。The aim of this work is to provide an accurate and scientific method of radionuclide imaging for small cell lung cancer with Tcm-2F7 F (99ab′ ) 2 . The monoclonal antibody 2F7 F (ab′ ) 2 segment was derived by iminothiolane and ligand exchange from Tcm O ( GH ) 99? 2 . Labeling efficiency was measured by paper chromatography. The label- ing efficiency of the labeled antibody 2F7 F ( ab′ ) 2 was 93.5%,with 2.5% colloidal Tcm in it. The competition test in 99 vitro showed that Tcm bound with the high affinity sites of antibody. The stability of the iminothiolane modified 99 antibody 2F7 F ( ab′ ) 2 could be kept at 4℃ for 3 months. In conclusions, the labelling method applied to the preparation of 99Tcm labeled antibody 2F7 F ( ab′ ) 2is successful, and appears to be equally applicable to other antibodies.

关 键 词：小细胞肺癌 单克隆抗体 放免显像 

分 类 号：R734.2[医药卫生—肿瘤] TL923[医药卫生—临床医学]