体胚发生途径‘大矮蕉’植株的再生  

Plant regeneration through somatic embryogenesis of Musa AAA cv. Grande Naine

作　　者：徐春香[1] B.PANIS H.STROSSE R.SWENNEN 李华平[1] 肖火根[1] 范怀忠[1] 

机构地区：[1]华南农业大学植物病毒研究室,广东广州510642 [2]比利时荷语鲁汶大学热带作物改良实验室

出　　处：《华南农业大学学报》2004年第2期63-66,共4页Journal of South China Agricultural University

基　　金：国家自然科学基金资助项目(39870434);广东省自然科学基金资助项目(990714);广东省科技攻关资助项目(C20209)

摘　　要：'大矮蕉'MusaAAAcv.GrandeNaine的胚性细胞悬浮系(ECS)在M2液体培养基中预培养1周和2周后,分别将其接种在RD1或M3培养基上,于光照或黑暗条件下进行体胚的再生.再生体胚在接种后3周左右开始出现,经8周的培养后,在不同预培养时间、再生培养基种类及培养条件下,胚性细胞(团)质量增长了约5～18倍,从沉积细胞体积(SCV)为1mL的ECS获得的再生体胚数为0 71×105～3 07×105.植株的再生率及从SCV为1mL的ECS获得的再生植株数也因上述体胚再生条件的不同而异.Embryogenic cell suspensions (ECS) of 'Grande Naine' (Musa AAA group) were plated on RD1 or M3 medium for the regeneration of somatic embryos, 1 to 2 weeks after last subculture. The first regenerable somatic embryos were observed about 3 weeks after inoculation. After 8 weeks of culture, the mass of the embryogenic mass increased about 5 to 18 times and the number of somatic embryos that could be regenerated from 1 mL settled cell volume (SCV) of ECS ranged between 0.71×10~5 and 3.07×10~5, depending on pre-culture time in liquid medium before regeneration, regeneration media and incubation conditions (light/dark). The frequency of plant recovery and the amount of plantlets regenerated from 1 mL SCV of ECS were indirectly affected by somatic embryos regeneration conditions that were studied.

关 键 词：体胚发生 大矮蕉品种 植株再生 胚性细胞悬浮系 培养基 香蕉 

分 类 号：S668.1[农业科学—果树学]