HLA衍生肽对大鼠脾细胞增殖功能及HO-1活性的影响  

Effects of HLA-derived peptide on proliferation and heme oxygenase-1 activity of rat spleen cells

作　　者：易善红[1] 宋波[2] 汪泽厚[3] 张艮甫[1] 崔志刚[4] 

机构地区：[1]第三军医大学新桥医院泌尿外科,全军肾脏病中心重庆400037 [2]西南医院全军泌尿外科中心,重庆400038 [3]空军总医院泌尿外科,北京100036 [4]总装备部医院泌尿外科,北京100042

出　　处：《第三军医大学学报》2004年第4期314-316,共3页Journal of Third Military Medical University

基　　金：国家新药基金资助项目 ( 1999A0 0 2 )~~

摘　　要：目的　观察一种新型组织相容性白细胞抗原 (histocompatibilityleukocyteantigen ,HLA)衍生肽 (RDP12 5 8)的免疫抑制功能并探讨其机制。方法　人工固相合成一种HLA衍生肽 (RDP12 5 8) ,用 3 H TdR法观察其在体外对大鼠脾细胞增殖反应的影响 ,以酶化学法观察其对脾细胞血红素氧合酶 1(hemeoxygenase 1,HO 1)活性的影响。结果　RDP12 5 8可显著抑制淋巴细胞转化及MLR的增殖反应 ;该肽体外降低HO活性呈现出剂量依赖性。结论　RDP12 5 8能够显著抑制大鼠脾细胞经丝裂原或同种抗原刺激引起的增殖反应 ,这种作用可能是通过影响HOObjective To investigate the immunosuppression function of a novel HLA derived peptide, RDP1258, and its mechanisms. Methods A peptide derived from HLA, RDP1258, was chemically synthesized by artificial solid phase synthesis. Effects of the peptide on alloreactive cytotoxic activity of rat spleen cells and heme oxygenase 1 (HO 1) activity were observed using 3H TdR method and enzyme chemistry method, respectively. Results The synthetic HLA derived peptide could obviously inhibit the proliferation of rat spleen cells and mixed lymphocyte reaction, and reduce HO activity in a dose dependent manner in vitro . Conclusion RDP1258 can significantly inhibit the proliferation of rat spleen cells induced by mitogen and isoantigen possibly by means of affecting HO 1 activity.

关 键 词：HLA衍生肽 混合淋巴细胞培养 血红素氧化酶-1 

分 类 号：Q516[生物学—生物化学] R322.21[医药卫生—人体解剖和组织胚胎学]