Morroniside protects cultured human umbilical vein endothelial cells from damage by high ambient glucose  

作　　者：Hui-qinXU Hai-pingHAO XuZHANG YangPAN 

机构地区：[1]CollegeofPharmacy [2]BasicMedicineCollege,NanjingUniversityofTraditionalChineseMedicine,Nanjing210029,China

出　　处：《Acta Pharmacologica Sinica》2004年第4期412-415,共4页中国药理学报（英文版）

基　　金：Projectsupported by theNatural Science Foundation of Jiangsu Province,No BK2001127.

摘　　要：AIM: To determine whether morroniside, a compound in Cornus officinalis Siebet Zucc can prevent cultured human umbilical vein endothelial cells (HUVEC) from damage by highambient glucose. METHODS: HUVEC was incubated in glucose, 5 or 30 mmol/L, either alone or in thepresence of morroniside (final concentration 100, 10, and 1 μmol/L, respectively) for 48 h. Theproliferation of HUVEC was quantified by MTT method; its cycle was analyzed by flow cytometry;morphological change was observed with fluorescence microscopy. RESULTS: Survival of HUVEC culturedin high ambient glucose was significantly decreased when compared to that in normal concentration ofglucose (P【0.01). High ambient glucose also lowered the rate of cells entering into S-phase, alongwith severe morphological damage. With the intervention of morroniside (final concentration 100 and10 μmol/L), the cell survival was significantly recovered (P【0.01, P【0.05, respectively),accompanied with increased S-phase rate and less extent of morphological damage. CONCLUSION:Morroniside protected HUVEC against high ambient glucose induced injury, which suggested thatmorroniside could exert a beneficial effect on preventing diabetic angiopathies.AIM: To determine whether morroniside, a compound in Cornus officinalis Siebet Zucc can prevent cultured human umbilical vein endothelial cells (HUVEC) from damage by highambient glucose. METHODS: HUVEC was incubated in glucose, 5 or 30 mmol/L, either alone or in thepresence of morroniside (final concentration 100, 10, and 1 μmol/L, respectively) for 48 h. Theproliferation of HUVEC was quantified by MTT method; its cycle was analyzed by flow cytometry;morphological change was observed with fluorescence microscopy. RESULTS: Survival of HUVEC culturedin high ambient glucose was significantly decreased when compared to that in normal concentration ofglucose (P<0.01). High ambient glucose also lowered the rate of cells entering into S-phase, alongwith severe morphological damage. With the intervention of morroniside (final concentration 100 and10 μmol/L), the cell survival was significantly recovered (P<0.01, P<0.05, respectively),accompanied with increased S-phase rate and less extent of morphological damage. CONCLUSION:Morroniside protected HUVEC against high ambient glucose induced injury, which suggested thatmorroniside could exert a beneficial effect on preventing diabetic angiopathies.

关 键 词：MORRONISIDE umbilical veins endothelial cells diabetic angiopathies 

分 类 号：R965[医药卫生—药理学] R587.1[医药卫生—药学]