灯盏花注射液对铜仿透析膜及内毒素诱生单个核细胞产生IL-1β转录及翻译的影响  

Effect of DengZhanHua injection and TongFang dialysis on endotoxin induced IL-1β transcription and interpretation in single nucleus cell

作　　者：邸阜生[1] 杨燕[1] 魏肃[1] 牟富荣[1] 卢奕[1] 

机构地区：[1]天津市第三中心医院,天津300170

出　　处：《天津中医药》2004年第2期111-113,共3页Tianjin Journal of Traditional Chinese Medicine

基　　金：天津市卫生局科研基金项目 (99KY40);天津市卫生局科技成果 (津20023095)

摘　　要：[目的]探讨中药灯盏花注射液对铜仿透析膜及内毒素诱生单个核细胞产生白介素 -1β(IL -1β)转录及翻译过程的影响 ,为中药灯盏花治疗IL -1β提供依据。[方法]单个核细胞与铜仿透析膜、内毒素及灯盏花共同培养 ,用生物活性法测定IL -1β,用RT -PCR法测定IL -1βmRNA。[结果]铜仿透析膜、内毒素所诱生的IL -1β与正常组相比较均有显著性差异 ,并且应用灯盏花后 ,均可对IL -1β产生不同程度的抑制 ;透析治疗组及非透析治疗组IL -1βmR表达可被灯盏花注射液抑制。[结论]铜仿透析膜、内毒素可以在转录及翻译水平诱生IL -1β。To discuss the effect of DengZhanHua injection and TongFang dialysis on endotoxin induced IL-1βtranscription and interpretation in single nucleus cell.Single nucleus cell,Tongfang dialysis membrane,lipopolysaccharide and Dengzhanhua were cultured together,the IL-1βwas determined by bioassay.Marked difference was observed when compared with normal group,DengZhanHua could inhibit IL-1βin different degree.The expression of IL-1βcould be inhibited in,dialysis therapeutic group and non-dialysis therapeutic group.[Conclusion]Tongfang dialysis membrane, lipopolysaccharide induce the IL-1βtranscription and interpretation.

关 键 词：灯盏花注射液 铜仿透析膜 内毒素 单个核细胞 IL-1Β 白介素-1β 血液透析 尿毒症 

分 类 号：R277.525[医药卫生—中医学]