井穴放血法对急性脑缺血大鼠缺血区脑组织凋亡相关蛋白的影响  

Influence of well acupoints blood-letting therapy on apoptosis associated protein of ischemic brain tissue in rats with acute cerebral ischemia

作　　者：王秀云[1] 李积胜[2] 刘公望[1] 郭义[1] 潘荣菁[1] 张果忠[2] 李桂兰[1] 韩煜[1] 王强[1] 

机构地区：[1]天津中医学院,天津300193 [2]武警医学院,天津300162

出　　处：《天津中医药》2004年第2期123-125,共3页Tianjin Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助 (No.30271641);天津市卫生局科研基金资助 (No.97Y107)

摘　　要：[目的]观察井穴放血法对急性缺血性脑损伤的脑保护作用。[方法]将实验大鼠分为假手术组、脑缺血组和脑缺血 +井穴放血治疗组 ,一侧大脑中动脉阻塞致急性缺血性脑损伤模型 (MCAO) ,治疗组在脑缺血后即刻给予井穴放血治疗 ,出血量为每穴1滴。分别于缺血后的1、3、6、24h处死 ,进行免疫组织化学染色 ,检测缺血区皮层脑组织c-fos蛋白 (FOS)及HSP70蛋白免疫阳性细胞 ,以观察井穴放血法对急性缺血性脑损伤后早期抑凋亡基因表达的影响。[结果]c-fos蛋白和HSP70蛋白在缺血区皮层脑组织表达增加 ,缺血后1hHSP70无表达 ,FOS表达增加 ,在随后观察的3、6、24h时程内HSP70和FOS均有随缺血时间延长表达增多的趋势。井穴放血治疗组在以上各时程c-fos及HSP70免疫阳性细胞数均高于同时段的缺血病模组 ,有统计学意义。[结论]急性脑缺血后应用井穴放血干预治疗可明显增强缺血区脑组织对抗神经细胞凋亡的即刻早期基因c-fos蛋白和抗应激HSP70蛋白的表达 ,从而提高了缺血后神经细胞对缺血、缺氧的耐受和适应能力 ,提高了缺血后神经元的可塑性 ,进而可影响晚期目的基因的表达 ,抵抗细胞凋亡的发展 ,增强脑修复能力。s: To observe the protective function of well acupoints blood-letting method against acute ischemic brain injury. Rats were divided into sham operation group,cerebral ischemia group and treatment group. Acute ischemic brain injury was made by middle cerebral artery obstruction(MCAO). Well acupoints blood-letting therapy ,one drip each time ,was given in rats in treatment group immediately after cerebral ischemia. 1,3,6 ,24 hours after ischemia,rats were killed and c-fos protein(FOS)and HSP70 immune positive cells of cortex tissue in ischemic region were determined by immunohistochemistry assay to observe the influence on anti-apoptosis gene expression at early stages of acute ischemic brain injury. Increased expression of c-fos protein and HSP70 protein in cortex tissue of ischemic region was observed with no expression of HSP70 1 hour after ischemia,while the expression of FOS was increased. Expression of FOS and HSP70 tended to increase with the time elapsed during the following 3,6,24 hours. The count of c-fos and HSP70 immune positive cells in treatment group was all higher than that in model group during the same period of time with statistical significance.[Conclusion] Well acupoints blood-letting therapy can obviously enhance the expression of c-fos and HSP70 protein in ischemic region,increase the ability against apoptosis and the tolerance and adaptation ability of nerve cell to hypoxia and ischemia ,and enhance the neuron compliance after ischemia,thus further influencing the expression of objective gene at late stages,resisting the development of apoptosis,enhancing the repairing ability of brain. It is suggested that well acupoints blood-letting therapy has certain protective action against early stroke.

关 键 词：井穴放血法 急性脑缺血 大鼠 脑保护 C-FOS蛋白 HSP70蛋白 

分 类 号：R277.733.1[医药卫生—中医学] R245.312