交联淀粉碘颗粒及其灭活病毒效果观察  

Observation of the morphology of cross-linked starch iodine and its virucidal efficacy

作　　者：李莉[1] 熊鸿燕[1] 张利军[1] 张跃[1] 

机构地区：[1]第三军医大学预防医学系流行病学教研室,重庆400038

出　　处：《第三军医大学学报》2004年第5期393-395,共3页Journal of Third Military Medical University

摘　　要：目的　检测交联淀粉碘 (CSI)对水溶液中病毒的灭活效果及影响因素 ,并观察消毒前后CSI的形态变化。方法　以辛德比斯病毒 (sindbis)和减毒的脊髓灰质炎病毒 (polio)作为实验病毒 ,以不同浓度的血浆作为有机物加入剂 ,通过细胞感染试验观察CSI对病毒的杀灭效果 ;用扫描电镜观察CSI颗粒在水溶液中消毒处理前后的形态变化。结果　CSI对溶液中sindbis和polio的灭活效果基本相同。在D hanks液中 ,当CSI的加入量为 5 0～ 10 0mg/ml ,作用 3 0～ 60min ,病毒灭活达 5～ 7logTCID50 ,CSI对病毒的灭活效果随CSI含量和作用时间的增加呈现增强的趋势 ;CSI对含 10 %血浆水溶液和血浆中病毒的灭活达 3～ 5logTCID50 ;在电镜下 ,CSI为 10 0 μm左右直径的呈圆形和椭圆形颗粒 ,经灭活病毒处理后 ,CSI颗粒大小无显著变化 ,但表面光滑度增加。结论　CSI对不含有机物水溶液中的脂质包膜病毒和非脂质包膜病毒有良好的灭活作用 ,但其灭活病毒的效果明显受溶液中有机物浓度的影响 ;Objective To detect the virucidal efficacy of cross linked starch iodine (CSI) and its influence factors in solution and to observe the morphological changes of CSI after disinfection. Methods Sindbis virus (Sindbis) and poliovirus (polio) were used as the test viruses, and different concentrations of plasm as the organic solvent. The virucidal efficacy of CSI was observed by means of cell infection test. The morphological changes of CSI particles after disinfection were observed by scanning electron microscopy. Results CSI had the same virucidal effect on Sindbis and polio viruses in solution. After treatment with CSI at a dose of 50-100 mg/ml for 30-60 min, the viruses were inactivated by 5-7 logs TCID 50 . The virucidal efficacy of CSI enhanced in dose and time dependent manners. CSI could inactivate the viruses in D hanks solution with 10% plasma or in plasma by 2-5 logs TCID 50 and 2-3 logs TCID 50 , respectively. Electron microscopy showed that the CSI was in the form of round and oval particles with the diameter of about 100 μm. After disinfection, the particle surface became more lubricious, but no morphological changes were observed. Conclusion CSI has effective virucidal effect on envelope viruses and non envelope viruses in solution free of organic substances, but the virucidal efficacy can be greatly affected by organic substances. There is no morphological change of CSI particles after disinfection.

关 键 词：交联淀粉碘 消毒 

分 类 号：R187[医药卫生—流行病学] R373[医药卫生—公共卫生与预防医学]