大劣按蚊AdS7基因的克隆及分析  

Cloning and analysis of AdS7 gene of Anopheles dirus

作　　者：郝宏兴[1] 黄复生[1] 段建华[1] 况明书[1] 王英[1] 

机构地区：[1]第三军医大学基础医学部病原生物学教研室,重庆400038

出　　处：《第三军医大学学报》2004年第6期476-478,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目 ( 30 2 0 0 2 37)~~

摘　　要：目的　克隆AdS7基因cDNA序列 ,为大劣按蚊基因定量研究提供内参照。方法　采用RT PCR扩增、TA克隆技术获得AdS7cDNA部分序列 ,标记地高辛AdS7探针 ,从大劣按蚊cDNA文库中筛选得到AdS7全长克隆 ,以半定量RT PCR方法研究血餐和约氏疟原虫感染对其表达水平的影响。结果　从大劣按蚊cDNA克隆得到长 464bp的AdS7基因cD NA部分序列 ,从大劣按蚊cDNA文库筛选获得了长 871bp的AdS7基因全长克隆 ,大劣按蚊吸血和感染约氏疟原虫后 2 4h ,AdS7表达水平没有显著变化。Objective To clone the cDNA sequence of ribosomal protein S7 of Anopheles dirus (AdS7) gene for providing an internal control gene for quantitative study of genes of Anopheles dirus . Methods Partial cDNA sequences of AdS7 were obtained by RT PCR and TA cloning. The full length clone of AdS7 was obtained by screening of the cDNA library of Anopheles dirus using digoxin labeled AdS7 probe. The effects of blood feeding and Plasmodium infection on the AdS7 expression were analyzed by semi quantitative RT PCR method. Results A cDNA sequence of AdS7 (464 bp) was cloned from the cDNA of adults of Anopheles dirus . The full length clone of AdS7 gene (871 bp) was obtained by screening of the cDNA library of Anopheles dirus . No change of AdS7 expression was observed at 24 h after blood feeding and Plasmodium infection. Conclusion AdS7 gene is a suitable internal control gene in the quantitative study of genes of Anopheles dirus .

关 键 词：AdS7 基因克隆 半定量RT-PCR 

分 类 号：Q554[生物学—生物化学] Q785