大劣按蚊对约氏疟原虫卵囊黑化相关血细胞类型的初步鉴定  

Preliminary identification of Anopheles dirus hemocytes related with anti-malaria melanization of Plasmodium yoelii oocysts

作　　者：杨松[1] 时超美[1] 周桃莉[1] 黄复生[1] 况明书[1] 王英[1] 

机构地区：[1]第三军医大学基础医学部病原生物学教研室,重庆400038

出　　处：《第三军医大学学报》2004年第6期498-500,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目 ( 30 30 0 2 92 )~~

摘　　要：目的　鉴定与抗疟黑化反应相关的大劣按蚊血细胞。方法　采用姬氏染色法初步鉴定大劣按蚊血细胞类型 ,继而利用免疫细胞化学技术检测大劣按蚊血细胞内前酚氧化酶和丝氨酸蛋白酶。结果　颗粒细胞和浆细胞为大劣按蚊主要血细胞 ,前酚氧化酶和丝氨酸蛋白酶也主要分布于颗粒细胞和浆细胞内 ,其中颗粒细胞最常见。Objective To identify the hemocytes related with anti malaria melanization response. Methods The types of hemocytes were identified preliminarily by Giemsa's staining, and then both prophenoloxidase (PPO) and serine protease (SP) were detected by immunocytochemical technique. Results The hemocytes were mainly granular cells and plasmatocytes in which Sp22D and PPO were distributed. Conclusion Hemocytes related with anti malaria melanization may mainly be granular cells and plasmocytes.

关 键 词：大劣按蚊 血细胞 黑化 免疫细胞化学 

分 类 号：R382.31[医药卫生—医学寄生虫学] R384.1[医药卫生—基础医学]