四肽AC-DEVD-CHO对硫芥诱导淋巴细胞凋亡的干预作用  

Interventional effect of tetrapeptide on sulfur mustard-induced lymphocyte apoptosis

作　　者：赛燕[1] 刘勇[1] 朱明学[2] 张黎明[2] 董兆君[1] 

机构地区：[1]第三军医大学预防医学系军事毒理学教研室,重庆400038 [2]第二军医大学海医系,上海200433

出　　处：《第三军医大学学报》2003年第14期1223-1226,共4页Journal of Third Military Medical University

基　　金：全军"十五"重点课题 ( 0 1L0 54 )

摘　　要：目的　探讨caspase 3活性在硫芥诱导淋巴细胞凋亡中的变化和其四肽抑制剂AC DEVD CHO对细胞凋亡的干预作用。方法　荧光分光光度法检测caspase 3活性变化 ,琼脂糖凝胶电泳和流式细胞仪分别检测DNAladder ,细胞凋亡率。结果　硫芥可诱导淋巴细胞caspase 3活性升高 ,其多肽抑制剂可部分阻断细胞凋亡 ,主要表现在抑制剂作用下DNAladder现象减弱 ,细胞凋亡百分数下降。结论　硫芥通过激活caspase 3诱导淋巴细胞凋亡 ,其多肽抑制剂对淋巴细胞凋亡具有干预作用。Objective To investigate the changes of caspase 3 activity and the interventional effect of tetrapeptide AC DEVD CHO on sulfur mustard induced apoptosis of lymphocyte. Methods The activity of caspase 3 was detected by fluorospectrophotometry. DNA ladder and apoptosis were detected by DNA agarose gel electrophoresis and flow cytometry. Results Sulfur mustard enhanced the activity of caspase 3 and its polypeptide inhibitor (AC DEVD CHO) partially blocked apoptosis, which was mainly presented as an obscure phenomenon of DNA ladder and a decrease in percentage of apoptosis. Conclusion Sulfur mustard can induce the apoptosis of lymphocyte by means of activating caspase 3. The inhibitor may have an interventional effect on lymphocyte apoptosis induced by sulfur mustard.

关 键 词：四肽 硫芥 淋巴细胞 细胞凋亡 胱氨酸蛋白酶-3 

分 类 号：R827.17[医药卫生—临床医学]