机械拉伸下人肺上皮细胞增殖及整联蛋白再分布  

Effects of mechanical strain on the proliferation of human pulmonary epithelial cells and reorganization of integrins

作　　者：张惠静[1] 杨力 蔡绍皙[3] 卢晓[3] 王远亮[3] 

机构地区：[1]第三军医大学医学检验系分析化学教研室,重庆400038 [2]Department of Bioengineering and Orthopedics,University of California,San Diego,CA 92093-0656,USA [3]重庆大学生物工程学院,生物力学与组织工程教育部重点实验室,重庆400044

出　　处：《第三军医大学学报》2003年第14期1254-1257,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金资助重点项目 ( 19732 0 0 3) ;高等学校重点实验室访问学者基金资助项目 ( 2 0 0 2 )

摘　　要：目的　探讨机械拉伸应变对人肺上皮细胞增殖及其膜受体———整联蛋白α5、β1 再分布的调控作用。方法　建立体外周期性拉伸装置 ,应用流式细胞术测定细胞的增殖活性 ,激光共聚焦显微镜分析整联蛋白α5、β1 再分布。结果　在应变为 15 % ,频率为 2 0次 min、40次 min的拉伸刺激下 ,2 4h后 ,细胞的增殖活性指数明显降低 ,H72 7细胞的DNA合成受到显著抑制。在 40次 min的拉伸频率下 ,整联蛋白α5、β1 的分布发生重组并向基底层转移 ,形成局部粘附连接。结论　整联蛋白α5、β1Objective To study the effects of mechanical strain on the proliferation of the human pulmonary epithelial cell and the redistribution of its membrane receptors, integrins α 5 and β 1. Methods A cyclic strain unit in vitro was designed. The cellular proliferative index was measured by flow cytometry and the redistribution of α 5 and β 1 integrins was analyzed in human pulmonary epithelial cell line H727 by laser confocal microscopy. Results The cellular proliferative index reduced significantly after cells were subjected to 15% elongation at frequencies of 20 cycles/min or 40 cycles/min for 24 h. In human pulmonary epithelial H727 cells, α 5 and β 1 integrins transferred from the apical layer to the basal layer and formed an adhesion plaque after 24 h exposure to 15% elongation at frequency of 40 cycles/min. Conclusion The results suggest that α 5 and β 1 integrins in pulmonary epithelial cells may play an important role in the transduction of mechanical stress.

关 键 词：机械应力 人肺上皮细胞 整联蛋白 粘着斑 

分 类 号：R318.01[医药卫生—生物医学工程] R322.35[医药卫生—基础医学]