ET-1、MMP-3、TIMP-1在人肾小球中的表达及意义  

The expressions of endothelin-1, matrix metalloproteinase-3 and tissue inhibitor of metalloproteinase-1 in human glomeruli

作　　者：黄志芳[1] 朱妙珍[1] 何娅妮[1] 申海鹰[1] 张建国[1] 杨聚荣[1] 

机构地区：[1]第三军医大学附属大坪医院野战外科研究所肾内科,重庆400042

出　　处：《第三军医大学学报》2003年第11期1009-1012,共4页Journal of Third Military Medical University

摘　　要：目的　研究内皮素 1(Endothelin 1,ET 1)、基质金属蛋白酶 3 (Matrixmetalloprotevnase 3 ,MMP 3 )、金属蛋白酶组织抑制剂 1(Tissueinhibitorofmelalloproteinase ,TIMP 1)在人肾小球中的表达变化及意义。方法　应用免疫组化技术检测3 2例原发性系膜增生性肾炎患者及 5例对照组肾小球中ET 1、MMP 3、TIMP 1、LN蛋白的表达。结果　在中、重度系膜增生性肾炎患者的肾小球中 ,ET 1、MMP 3、TIMP 1、LN蛋白表达增强 ,MMP 3 /TIMP 1比值下降 (P <0 0 5 ,P <0 0 1) ;ET 1的表达与MMP 3 /TIMP 1比值负相关 (r =-0 70 3 ,P <0 0 1) ,与LN表达正相关 (r =0 880 ,P <0 0 1) ;MMP 3 /TIMP 1比值与LN表达负相关 (r=-0 62 3 ,P <0 0 1)。结论　ET 1可能是肾小球MMP 3 /TIMP 1下降的原因之一 ,这与肾小球细胞外基质 (Exlracellularmatrix ,ECM)降解受抑密切相关。Objective To investigate the expressions of endothelin 1(ET 1), matrix metalloproteinase 3(MMP 3) and tissue inhibitor of metalloproteinase 1(TIMP 1) in human glomeruli and the related significance. Methods A total of 32 patients with primary mesangioproliferative glomerulonephritis(MsPGN) and 5 controls were included in this study. The expression levels of ET 1, MMP 3, TIMP 1 and LN proteins were determined by immunohistochemistry. Results The expressions of ET 1, MMP 3,TIMP 1 and LN proteins enhanced in normal glomeruli of moderate and severe MsPGN. The ratio of MMP 3/TIMP 1 decreased in severe MsPGN( P <0.05 or P <0.01). The expression of ET 1 was negatively correlated with the ratio of MMP 3/TIMP 1( r =-0.703, P <0.01) but positively with the expression of LN( r =0.880, P <0.01). The ratio of MMP 3/TIMP 1 was negatively correlated with the expression of LN( r =-0.623, P <0.01). Conclusion ET 1 may be involved in the decrease of the ratio of MMP 3/TIMP 1, which may contribute to the inhibition of ECM degradation in human glomeruli.

关 键 词：内皮素-1 基质金属蛋白酶 金属蛋白酶组织抑制剂 

分 类 号：R322.61[医药卫生—人体解剖和组织胚胎学] R341.6[医药卫生—基础医学]