IL-1B-31及IL-1RN基因多态性与胃癌易感性关系  

Relationship Between Polymorphisms Interleukin-1 B-31 and Interleukin-1 Recepetor An tagonist Gene and Susceptibility to Gastric Cancer

作　　者：杨婕[1] 沈洪兵[1] 沈靖[1] 钮菊英[1] 胡志斌[1] 徐耀初[1] 

机构地区：[1]南京医科大学流行病与卫生统计学教研室,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2004年第3期193-197,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助项目(30170827)

摘　　要：目的:研究白细胞介素1B基因(IL-1B)启动子区域-31位点和白细胞介素1受体拮抗剂基因(IL-1RN)多态性在中国南方人群胃癌患者与健康对照中的分布,初步探讨其各基因型与胃癌的关联性。方法:收集285例胃癌患者与265例健康人群的外周血标本和流行病学调查资料,提取基因组DNA,IL-1RN基因型采用PCR方法直接确定。IL-1B基因采用PCR-RFLP方法分型。结果:IL-1RN基因有5种基因型,分别是1/1、1/2、1/3、1/4、2/2型,其频率在病例组中分别为81.9％、14.6％、7.0％、1.8％和1.1％。而在对照组中分别为81.6％、16.5％、0、0.8％和1.2％。各基因型在病例与对照组中分布没有显著性差异。IL-1B基因-31位点3种基因型C/C、C/T、T/T在胃癌病例组中频率分别为21.8％、48.6％和29.6%。而在对照组中分别为27.9％、48.8％和23.3％。与C/C型比较,携带T/T基因型者胃癌发生的危险性增加63.3％(OR=1.633,95％.CI=1.014-2.628)。这一危险性在高年龄组、男性、饮酒、HP感染阳性、有癌症家族史的人中更为显著。结论:IL-1B基因启动子区域-31位点多态性可能与该人群胃癌易感性相关;未发现IL-1RN基因多态性与胃癌易感性关联。Objective: To evaluate the frequency of interleukin-lB(IL-lB) promoter region -31 allele and interleukin-1 receptor antagonist gene (IL-1RN) polymorphisms in the gastric cancer case and controls in a Chinese population , and to elucidate the relationship between the genotype of IL-1B-31 and IL-1RN and susceptibility to gastric cancer. Methods: Blood samples from 285 patients with gastric cancer and 265 age and sex matched controls were collected. Genomic DNA was extracted and subjected to PCR amplification for judging IL-1RN genetype. The PCR product was digested by restriction endonuclease Alu Ⅰ for judging IL-1B genetype. Results: Five kinds of polymorphism of IL-1RN were found as 1/1, 1/2, 1/3, 1/4, 1/5 and the frequency in patients were 81. 9%, 14. 6%, 7. 0%, 1. 8% and 1. 1% , respectively. However, the frequency in normal control were 81. 6% , 16. 5%, 0 , 0. 8% and 1. 2%. No significant differences were observed between cases and controls in each genotype. The polymorphisms of IL-1B -31 allele were C/C,C/T, T/T. The frequency in patients were 21.8% , 48. 6% and 29. 6% and the frequency in normal control were 27. 9%, 48. 8% and 23. 3% . IL-1B-31T/T carriers were at an increased risk of gastric cancer with an odds ratio of 1. 633 (95% CI, 1.014-2. 628), especially with those older than 60, male, positive of HP infection and having a family history of cancer and habit of drinking. Conclusion: In south Chinese population, the polymorphism of interleukin-1B (IL-1B) promoter region 31 allele may be associated with the susceptibility of gastric cancer. However, no evidence was found to support that the polymorphism of IL-1RN had relationship with gastric cancer.

关 键 词：IL-1 基因多态性 胃癌 

分 类 号：Q305[生物学—遗传学] R181.34[医药卫生—流行病学]