癌痛平胶囊抗癌作用的实验研究  

Experimental Research on Anti-cancer Effect of Aitongping Capsule

作　　者：吴勉华[1] 许惠琴[1] 周红光[1] 

机构地区：[1]南京中医药大学,江苏南京210029

出　　处：《南京中医药大学学报》2004年第3期162-164,共3页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏省中医药局资助项目 (970 0 4)

摘　　要：目的　分别观察癌痛平胶囊对HAC肝癌小鼠的抗癌作用及体外对人肝癌细胞株SMMC 772 1的细胞增殖反应的抑制作用。方法　①无菌条件下于KM雌性小鼠右腋下接种HAC肝癌细胞 ,随机分为 4组 ,每日灌胃给药 ,连续 8日。末次给药后次日 ,测定小鼠体重、白细胞数以及小鼠肿瘤、脾脏和胸腺重量 ,用光镜检查肿瘤组织 ,并用图像处理系统处理并计算肿瘤坏死的程度。②培养人肝癌细胞株SMMC 772 1,随机分为 5组 ,并分别给药 ,用噻唑蓝还原法 (MTT法 )测定SMMC 772 1细胞增殖反应。结果　癌痛平Ⅰ、Ⅱ组均能减轻HAC肝癌小鼠的癌重 ,其抑癌率分别达到 6 4 .0 %和 5 2 .3% ;癌痛平能提高HAC肝癌小鼠癌组织的坏死程度 ,与模型组比较具有显著性差异 (P <0 .0 1)。癌痛平Ⅲ组能减少SMMC- 772 1细胞线粒体MTT代谢产物吸收度值 (A值 ) ,与空白对照组比较具有显著性差异 (P <0 .0 1)。结论　癌痛平能明显减轻HAC肝癌小鼠的癌重 ,提高HAC肝癌小鼠癌组织的坏死程度。体外实验亦显示癌痛平对人肝癌细胞株SMMC 772 1的细胞增殖反应具有显著抑制作用。OBJECTIVE To observe the anti-cancer effect and inhibiting effect of Aitongping Capsule. METHOD (1) HAC liver cancer cells were inoculated to mice, which were divided into 4 groups and were given intragastric administration with Aitongping Capsule for 8 days. On the ninth day, the mice were weighed, white cell count and the weights of tumor, spleen and thymus were determined, the tumor tissue were examined by microscope and the degree of necrosis was calculated. (2) Human liver cancer cell strains SMMC-7721 were cultivated and divided into 5 groups randomly. MTT method was used to determine SMMC-7721 cell proliferation. RESULT In Aitongping groups I and II, the cancer-inhibiting rate were 64.0% and 52.3% respectively and cancer tissue necrosis rate was increased, with significant difference as compared with the control groups ( P <0.01). In Aitongping group III, the absorption rate (A value) of MTT metabolism product was reduced, with significant difference as compared with the control groups ( P <0.01). CONCLUSION Aitongping Capsule can markedly reduce the weight of cancer tissue and increase the degree of necrosis of cancer tissue. In vitro experiments have shown that Aitongping Capsule has marked inhibiting effect on SMMC-7721 proliferation, demonstrating its satisfactory anti-cancer effect.

关 键 词：癌痛平胶囊 抗癌 实验研究 

分 类 号：R285.5[医药卫生—中药学]