幽门螺杆菌黏附素基因babA_2的克隆表达及定位  

Cloning high expression and location of H.pylori adhesion babA_2 gene

作　　者：白杨[1] 王继德[1] 陈烨[1] 林焕建[1] 张兆山[2] 张亚历[1] 

机构地区：[1]第一军医大学南方医院全军消化病研究所,广东广州510515 [2]军事医学科学院生物工程研究所,北京100071

出　　处：《第四军医大学学报》2004年第2期128-130,共3页Journal of the Fourth Military Medical University

基　　金：国家 8 63计划专题 (1 0 2 0 7 0 3 0 6);国家自然科学基金(30 1 70 890 );军队"十五"医药卫生科研课题 (OIMA 1 32 )资助项目

摘　　要：目的 :克隆并表达H .pylori黏附素babA2 基因 .方法 :提取H .pylori染色体基因 ,用PCR方法扩增babA2 基因 ,将其克隆至表达载体pET 2 2b(+) ,并在BL2 1 (DE3)大肠杆菌中表达及定位分析 .结果 :分离得到了 2 .2kb的babA2基因片段 ,在 37℃诱导表达 3h后 ,重组蛋白表达量占菌体总蛋白的 34.8% ,其中分泌表达占周质总蛋白的 2 2 .7% ,可溶性表达占上清的 1 5 .0 % ,包涵体占沉淀的 86 .7% .结论 :H .py loribabA2 基因的克隆与表达为H .AIM: To isolate and express bab A 2 gene from H.pylori. METHODS: bab A 2 gene was amplified from H.pylori chromosome by PCR and inserted into the prokaryotic expression vector pET 22b(+).The recombinant plasmid was transformed into the BL21(DE3) E.coli strain. RESULTS: The 2.2 kb bab A 2 gene was successfully isolated. Recombinant E.coli strains expressed bab A 2 were obtained, the expression protein amounted to 34.8% of the total bacterial protein , 22.7% of the bacterial periplasm protein , 15.0% of the bacterial sonicate supernatant and 86.7% of the bacterial inclusion body after being induced with IPTG for 3 h at 37℃. CONCLUSION: Cloning and high expression of bab A 2 gene lay the foundation for further research in the molecular mechanism of H.pylori adhesin and constructing the H.pylori vaccine.

关 键 词：螺杆菌 幽门 黏附素6n6A2 克隆 分子 基因表达 

分 类 号：R378.2[医药卫生—病原生物学]