商陆抗病毒蛋白体外对HepG2.2.15细胞HBV复制的影响  

The effects of pokeweed antiviral protein from seeds on the replication of hepatitis B virus in vitro

作　　者：贺永文[1] 潘延凤[1] 王萍[1] 郭劲松[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院传染病学教研室,武汉市430022

出　　处：《实用肝脏病杂志》2004年第2期80-82,共3页Journal of Practical Hepatology

基　　金：湖北省自然科学基金资助 ;No.2 0 0 1ABB1 4 8

摘　　要：目的　探讨商陆抗病毒蛋白 (pokeweedantiviralprotein ,PAP)体外抗乙型肝炎病毒 (HBV)的活性。方法　以不同浓度的PAP S作用于培养的HepG2 .2 .15细胞 ,采用ELISA、荧光定量PCR法分别检测细胞上清液中的HBsAg、HBeAg及HBVDNA水平的变化 ,并用MTT比色法检测细胞成活率。结果　随着PAP S浓度的增加 ,对HBsAg、HBVDNA的抑制作用亦逐渐增强 ,不同药物剂量组之间差异十分显著 (P <0 .0 1)。PAP S处理后 96h ,PAP S对HBsAg和HBeAg的半数抑制浓度 (ID50 )分别为 6.9μg/ml和 3 .2 μg/ml ,半数中毒浓度 (CD50 )为 2 9.3 μg/ml,治疗指数 (TI) =ID50 /CD50 分别为 4.3和9.3。MTT试验结果显示 ,药物的细胞毒性也呈剂量依赖型 ,倒置显微镜下观察在 5 0 μg/ml～ 10 0 μg/ml剂量组出现部分细胞脱壁和死亡现象。结论　PAP S在体外有直接的抗HBV作用。。Objective To probe the effects of pokeweed antiviral protein from seeds (PAP S) on hepatitis B virus replication in vitro.Methods HepG2.2.15 cells in cultured medium were treated with different concentrations of PAP S.HBsAg,HBeAg and HBV DNA in supernatants were determined by ELISA and fluorescent quantified PCR respectively.MTT method was used to assay the cytotoxity of PAP S.Results The results showed that the inhibited effect of PAP s on HBsAg,HBeAg and HBV DNA was gradually enhanced with increasing PAP concentration.The difference of HBsAg,HBeAg and HBV DNA levels was significant among different dose groups at 96h after PAP S treatment (P<0.01).The median inhibition dose of PAP S for HBsAg/HBeAg expression (ID 50 ) was 6.9μg/ml and 3.2 μg/ml,respectively;and the median cytotoxic dose (CD 50 ) was 29.3μg/ml.The therapeutic index for HBsAg/HBeAg were 4.3 and 9.3 at 96h respectively.However,cytotoxic experiment results showed that cytotoxicity of PAP S for cells increased with increasing PAP S concentration.When PAP S concentration reached 50μg/ml～100μg/ml,it resulted in a part of cells to falling off the well or death.Conclusion PAP S has strong inhibitory effect on HBV in vitro.

关 键 词：商陆抗病毒蛋白 核糖体失活蛋白 乙型肝炎病毒 HEPG2.2.15细胞 

分 类 号：R285[医药卫生—中药学]