真菌诱导子对胡桐悬浮培养细胞产生红厚壳素的影响  

Effects of fungal elicitor on inophyllums production in suspension cultured cells of Calophyllum inophyllum L.

作　　者：罗焕亮[1] 郭勇[1] 崔堂兵[1] 代建国[2] 张俊松[2] 许柏球[2] 

机构地区：[1]华南理工大学食品与生物工程学院,广东广州510641 [2]深圳职业技术学院生物应用工程系,广东深圳518055

出　　处：《药学学报》2004年第4期305-308,共4页Acta Pharmaceutica Sinica

摘　　要：目的　探讨真菌诱导子对胡桐悬浮培养细胞产生红厚壳素的影响。方法　通过对胡桐叶斑病病菌的分离培养 ,制成真菌诱导子补加到胡桐细胞悬浮培养基中 ,考察不同浓度、不同加入时间对胡桐细胞生物量及红厚壳素产量的影响。结果　S I菌株诱导胡桐CR2细胞合成红厚壳素的最佳浓度为 60mgGE·L- 1,诱导子在细胞生长静止期初期 (即培养d 18)加入对红厚壳素产量影响最大 ,可使产量提高 2 7% ,并促进了红厚壳素向胞外分泌。AimTo investigate the effects of fungal elicitors on inophyllums production in susp ension cultured cell of Calophyllum inophyllum Linn. MethodsThe pathogen of leaf spot disease of C.inophyllum L. was isolated and prepar ed as fungal elicitor. The fungal elicitor was added to the medium with differen t concentrations and culture period. Their effects on biomass and inophyllums co ntent of the suspension of cultured cells were studied. ResultsThe optimum effects of S-I fungal elicitor concentrations on inophyllums conten t was 60 mg GE·L -1 . Adding the fungi elicitor into the cell suspensio n culture system at stationary phase (being cultured for 18 days) resulted in a highest inophyllum content of 59 174 mg·L -1 at the 3rd day with 27% higher than control. Fungal elicitor treatment promoted the inophyllums accumula tion in medium. ConclusionAdding the Stagonospora curtisii (Berk.) Sacc.to the medium was effective ap proaches to enhance inophyllums yield in the suspension of C.inophyllum L cu lture cell.

关 键 词：真菌诱导子 胡桐 悬浮培养细胞 红厚壳素 影响因素 

分 类 号：R282.71[医药卫生—中药学]