棕色田鼠和沼泽田鼠雄性社会行为与嗅觉相关脑区性激素受体表达之间的关系  

The relationship between social behavior and the expression of estrogen receptor β and androgen receptor in olfactory-related brain regions of the male mandarin vole Microtus mandarinus and reed vole M. fostis

作　　者：何凤琴[1] 邰发道[1] 张育辉[1] 安书成[1] 

机构地区：[1]陕西师范大学生命科学学院,西安710062

出　　处：《动物学报》2004年第2期165-175,共11页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金资助项目 (No .3 0 2 0 0 0 2 6);教育部科学技术重点项目~~

摘　　要：应用行为聚焦取样观察和免疫组织化学相结合的方法 ,比较研究了棕色田鼠 (Microtusmandarinus)(n =15 )和沼泽田鼠 (M .fostis) (n =15 )在同种雄雄交往中的行为差异 ,及在雄雄交往前后雌激素 β受体(ERβ)和雄激素受体 (AR)表达的差异。在 2h的雄雄交往中 ,前 1h棕色田鼠对同性入侵者有较多的攻击和防御行为 ,后 1h攻击行为较少 ,沼泽田鼠前后 1h差异不大 ,整个 2h期间 ,棕色田鼠较沼泽田鼠对同性入侵者有较多的攻击、防御行为 ,较少的非社会行为。经过免疫组织化学检测 ,没有社会交往时棕色田鼠主嗅球系统投射区和犁鼻系统投射区ERβ免疫阳性细胞 (ERβ IRs)明显少于沼泽田鼠 ,且显色淡 ,AR免疫阳性细胞(AR IRs)在两种鼠间差异不大 ,且都明显少于各自的ERβ IRs。 2h交往后 ,棕色田鼠主嗅球投射区和犁鼻系统投射区的ERβ IRs细胞数明显少于交往前 ,AR IRs细胞数明显多于交往前 ;沼泽田鼠交往前与交往后ERβ IRs和AR IRs细胞数均无显著差异 ,且显著多于交往后棕色田鼠ERβ IRs细胞数 ,显著少于交往后棕色田鼠AR IRs细胞数。以上结果表明 :两种田鼠在社会交往中社会行为不同 ;ERβ的减少和AR的增多可能在社会识别及攻击行为中均起一定的作用 。In order to investigate the neurobiological mechanism of the social behavior difference in adult male mandarin voles Microtus mandarinus and reed voles M. fostis, the behavior focal sampling method and immunohistochemistry method were used to investigate differences in intraspecies male-male interactions and the expressions of estrogen receptor β(ERβ) and androgen receptor(AR) between two species, respectively(n=15). During the two-hour interaction of male individuals, mandarin voles spent significantly more time on aggressive and defensive behavior to homosexual invaders in the first one hour than in the last hour, whereas the reed voles did not have significantly behavioral difference between the first and last hour. During the whole two-hour interaction of male individuals, mandarin voles spent significantly more time engaged in aggressive and defensive behaviors to homosexual invaders and less time on the nonsocial behaviors than the reed voles. Before the two-hour interaction of male individuals,we found obviously fewer and lighter-stained ERβ immunoreactive neurons (ERβ-IRs) in both main olfactory bulb system project regions and vomeronasal system project regions in the mandarin voles than in the reed voles,but no significant difference in numbers of AR immunoreactive neurons(AR-IRs) between the two species through the examining of immunoreactive neurons. Moreover,there were significantly fewer AR-IRs than ERβ-IRs in two species of vole before the two-hour interactions. There were significantly fewer ER-IRs in both the main olfactory bulb project regions and the vomeronasal system project regions in the mandarin voles after interactions than before interactions,but there were significantly more AR-IRs after interactions than before interactions in the mandarin voles. Differences in both numbers of ERβ-IRs and AR-IRs between before and after interactions in the reed voles were not significant. There were greatly significantly more ERβ-IRs neurons in reed voles than in mandarin voles which had gone t

关 键 词：棕色田鼠 沼泽田鼠 雄性 社会行为 嗅觉 主嗅球系统 犁鼻系统 雌激素Β受体 

分 类 号：S443[农业科学—农业昆虫与害虫防治]