玉米不同杂种优势近等基因系及其杂交种的基因表达分析  

Gene Expression Analysis of Near-lsogenic Lines for Different Heterosis in Maize

作　　者：王泽立[1] 王鲁昕[1] 张永慧 马俊业[1] 

机构地区：[1]山东农业大学生命科学学院,泰安271018 [2]莱州农业科学院,莱州261417

出　　处：《Acta Genetica Sinica》2004年第4期345-348,共4页

基　　金：国家留学基金资助 (编号 :993 0 70 45 )~~

摘　　要：以自发突变产生的籽粒大小和穗位叶片不同的 3对近等基因系及其杂交种为材料 ,研究了 6个基因在其叶片组织中的表达情况 ,以评价这些基因在杂种优势形成中可否作为“限率性基因”模式存在。结果表明 ,有些基因通过增强或减弱表达在杂种优势现象中起一定作用。将被测基因视为整体时会发现它们依次增强表达趋势为 :近等基因系 4 88 1>4 88 2 >4 78;相应杂交种 4 88 1× 340 >4 88 2× 340 >4 78× 340。对近等基因系及其杂交种个体千粒重和穗粒重的相关分析表明 ,Sus1、Sh1和ZmSps34 3′基因亦可作为适合的限率性候选基因以研究杂种优势分子机理。In this report,we used three pairs of near-isogenic lines(NILs) 478,488-1 and 488-2,which are different in seed size and leaves above the top ear,to make a survey of the expressions of six genes in leaf tissues of these NILs series in order to find whether these genes serve as models for the rate-limiting genes in the heterosis.Our results showed that some of genes may play a role in heterosis response either through increased or decreased expression.When all six genes were considered as a whole,they showed a tendency for higher gene expression in 488-1 than in 488-2 and in 478,and in 488-1×340 than in 478×340 and in 488-2×340.Sus1,Sh1 and ZmSps34-3′ were good candidate genes because they have been proposed to be rate-limiting for the seed size and grain weight of the individuals heterosis.And also,in order to seek a molecular genetic dissection of heterosis,two main theories,dominance and overdominance,which hold the classical explanation for the heterosis,were discussed too.

关 键 词：玉米 近等基因系 杂种优势 基因表达 

分 类 号：S513[农业科学—作物学]