反义c-fos核酸对自体移植静脉内膜增生的影响  

The effects of c-fos antisence olioneucleotide on the intimal proliferation of autogrfted veins

作　　者：王春喜[1] 孙勇[1] 王琦[2] 梁发启[1] 

机构地区：[1]中国人民解放军总医院血管外科,北京100853 [2]吉林大学第三医院血管外科,吉林长春1300031

出　　处：《中国现代普通外科进展》2004年第2期109-111,共3页Chinese Journal of Current Advances in General Surgery

基　　金：中国博士后科学基金资助项目 (中博基 [1999] 17)

摘　　要：目的 :利用反义核酸技术和显微外科技术 ,探讨反义c fos核酸对自体移植静脉内膜增生的影响。方法 :选择 2 0只新西兰家免 ,等分为实验组和对照组 ,均行自体颈外静脉、颈总动脉移植手术 ,实验组移植静脉周围和血管吻合口周围应用反义c fos核酸凝胶涂布 ;对照组仅行凝胶涂布。术后 2周取出移植血管 ,分别行病理学、免疫组织化学检测移植血管内膜厚度 ,内膜平滑肌细胞数及PCNA阳性表达情况。结果 :实验组移植血管内膜厚度、管腔狭窄度及VSMC数均较对照组减少 ,PCNA阳性表达情况亦较对照组明显减少。结论 :反义c fos核酸可有效的抑制移植静脉内膜的增生 。Objective:To investigate the effects of c-fos antisence olioneucleotide on the intimal proliferation of autogrfted veins with antisence technology.Methods:The external jugule veins were grafted into common carotid arteries in 20 rabbits and were divided into test group and control group randomly.The anastomosis and transplanted vein were coated with c-fos antisence olioneucleotide glue gel in the test group,while the contral group were merely coated with glue gel.The autografted veins were removed and measured by means of pathology and immunohistochemistry two weeks later.Results:The results show that the thickness of the venous intima,the degree of the vascular stricture,the expression of PCNA and the numbers of vascular smooth muscle cell were decreased in test group.Conclusion:The results suggest the c-fos antisence olioneucleotide can inhibit the intimal proliferation of the autografted veins.It is a prospective and idea genetic prophylactic therapy to intimal hyperplasia.

关 键 词：基因 C-FOS 寡核苷酸类 反义 血管内膜 移植 自体 静脉 

分 类 号：R543.6[医药卫生—心血管疾病]