造血因子动员后自体外周血干细胞移植对急性放射病猴的治疗作用  

Effect of hematopoietic factors mobilized peripheral blood stem cells on rhesus monkeys after irradiation of 7.0Gy γ ray

作　　者：熊国林[1] 郝静[1] 黄海潇[1] 谢玲[1] 郭德煌[1] 柳晓兰[1] 赵振虎[1] 王欣茹[1] 陈松森[2] 雒蓬轶 罗庆良[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850 [2]中国医学科学院基础医学研究所 [3]成都地奥九泓药厂

出　　处：《解放军医学杂志》2004年第4期330-332,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家"8 63"高技术基金(编号 2 0 0 1AA2 1 540 1 );全军"十五"指令基金 (编号 0 1L0 1 9)资助课题

摘　　要：目的　观察重组人粒细胞集落刺激因子 (rhG CSF)或rhG CSF +重组人干细胞因子 (rhSCF)动员后外周血干细胞 (PBSC)自身移植对 7 0Gyγ射线照射猴的治疗效果。方法　 15只正常成年猕猴分成照射对照、rhG CSF和rhG CSF +rhSCF 3组。rhG CSF 10 μg/(kg·d) (μKD)连续 5天皮下注射 ,rhSCF 2 0 μKD连续 8天皮下注射 ,后 5天与rhG CSF联用。照射对照组注射等体积的生理盐水。照射当天收集PBSC ,7 0Gyγ射线照射后 3～4h做自体PBSC移植 ,观察其造血恢复情况。结果　在移植后恢复期rhG CSF +rhSCF组白细胞数明显高于对照组和rhG CSF组。rhG CSF+rhSCF和rhG CSF组外周血小板数的恢复优于照射对照组 ,骨髓CFU数明显高于对照组。病理组织学检查表明 ,rhG CSF组骨髓腔内造血细胞数量超过照射对照组 ,但比rhG CSF +rhSCF组少。结论　rhG CSF或rhG CSF +rhSCF组动员后自体PBSC移植可明显促进急性放射病猴的造血功能恢复 ,两因子联用组的效果优于单因子组。Objective To observe the curative effect of rhG-CSF or rhG-CSF+rhSCF mobilized peripheral blood stem cells (PBSC) on rhesus monkeys after irradiation of 7.0Gy γ ray.MethodsFifteen healthy adult rhesus monkeys were divided into placebo control, rhG-CSF, and rhG-CSF+rhSCF mobilized groups. rhSCF 20μg·kg -1 ·d -1 (μKD) was administered for 8 consecutive days. Then from the 4th day to the 8th day, animals of both rhG-CSF and rhG-CSF+rhSCF mobilized groups were also given rhG-CSF 10μKD. The placebo control animals were given 0.9% sodium chloride of the same volume per kilogram. On day 0 of the irradiation, PBSCs were collected from all subjects. 3～4 hrs after irradiation, all animals received autologous PBSC infusion. ResultsFrom day 21 to day 29 after TBI, rhG-CSF+rhSCF mobilized group showed higher WBC counts compared to placebo control and rhG-CSF mobilized group. Platelet counts of both mobilized groups recovered more quickly than those of placebo control. Bone marrow nucleated cells culture demonstrated that rhG-CSF and rhG-CSF+rhSCF mobilized PBSC had stimulated bone marrow nucleated cells to form more CFUs. Histopathological evaluation showed the number of hematopoietic cells in the bone marrow of rhG-CSF mobilized group was greater than those in placebo control but less than those in rhG-CSF+rhSCF mobilized group. ConclusionThe infusion of rhG-CSF or rhG-CSF+rhSCF mobilized PBSC can improve the hematopoietic recovery in rhesus monkeys after 7.0Gy γ ray irradiation. The combined use of two mobilizing factors gives better result than using single factor.

关 键 词：粒细胞集落刺激因子 重组 干细胞因子 辐射损伤 外周血干细胞移植 猕猴 

分 类 号：R818.05[医药卫生—放射医学]