血型单克隆抗体试剂规模化生产工艺的建立  

Establishment of process to produce blood grouping reagents(monoclonal antibody) in a large scale

作　　者：杨福军[1] 沈荣[1] 张兰香[1] 吴菊寿[1] 任雅萍[1] 席仲兴[1] 彭林峰[1] 张正雷[1] 

机构地区：[1]兰州生物制品研究所,兰州730046

出　　处：《微生物学免疫学进展》2004年第2期22-24,共3页Progress In Microbiology and Immunology

摘　　要：为了满足规模化生产的需要 ,对血型杂交瘤细胞的接种量、培养时间、培养条件等重要步骤进行了优化比较。结果表明 ,最佳血型细胞的接种量为 2 4× 10 8个 ,过长上清的凝集效价达到 1∶12 8,重复性好。建立了切实可行的血型试剂大规模生产工艺 。The optimal condition for preparation of blood grouping reagents(monoclonal antibody)in a large scale was reported,certain important steps were compared and optimized including the quantity of cell inoculation, duration of culture time and conditions related to cell growth. The results showed that the suitable cell quantity for inoculation was 24×10 8 per bottle ,agglutination titer of overgrowth culture supernatant was 1∶128.Thus the most optimal production process for blood grouping reagents was set up and the preparation with stable quantity was produced.

关 键 词：血型试剂 工艺 单克隆抗体 

分 类 号：TQ464[化学工程—制药化工]