从半合成噬菌体抗体库筛选抗狂犬病毒人单链抗体  

Screening of Human Anti-rabies ScFv from A Phage Display Antibody Library

作　　者：闭兰[1] 张爱华[1] 彭祥兵[1] 王志友[1] 张智[1] 余模松[1] 

机构地区：[1]武汉生物制品研究所,武汉430060

出　　处：《中国生物制品学杂志》2004年第2期65-67,共3页Chinese Journal of Biologicals

摘　　要：目的　应用纯化的狂犬病毒抗原从半合成噬菌体抗体库中筛选针对狂犬病毒的人单链抗体(ScFv)。方法　用固相化的狂犬病毒抗原对半合成抗体库进行 3轮“吸附 洗脱 扩增”的筛选 ,从第 3轮洗脱下来的克隆中获得一株有可溶性表达且特异性结合狂犬病毒抗原的ScFv ,并进行基因序列测定。结果　所获氨基酸序列经blast数据库搜索 ,与一种抗狂犬病毒免疫球蛋白的氨基酸序列同源性最高 ( 82 % )。经检索kabat数据库 ,发现其轻、重链可变区分别属于VkⅠ型、VHⅢ型。结论　从噬菌体抗体库可以方便快捷地分离到针对狂犬病毒的单链抗体 。Objective To screen anti rabies ScFv from a semi synthetic phage antibody library using purified rabies virus antigen.Methods Screen anti rabies ScFv from a semi synthetic phage antibody library with solid rabies virus antigen by 3 cycles of “absorption eluction amplication” to obtain a clone which can react with rabies virus antigen and be used for the expression of soluble ScFv.Identify the clones by sequencing.Results The homology of the amino acid sequence of the obtained clone to that in blast data bank was 82%.By comparing with the sequences of variable regions of heavy and light chains in kabat data base,the obtained clone was found carrying a ScFv encoding gene of VkⅠ and VHⅢ types.Conclusion The antirabies ScFv carrying clone was isolated rapidly from the phage display antibody library and the antibody may be useful in preventing rabies virus infections.

关 键 词：狂犬病毒 人单链抗体 噬菌体抗体库 预防 免疫球蛋白 

分 类 号：R373.9[医药卫生—病原生物学]