无血清培养基与含血清培养基培养CHO细胞的比较  

Culture of CHO Cells in Serum-free and Serum-containing Media

作　　者：付百年[1] 赵睿[1] 王智宇[1] 王佳凤[1] 李德娟[1] 金立杰[1] 

机构地区：[1]长春生物制品研究所,长春130062

出　　处：《中国生物制品学杂志》2004年第3期158-160,共3页Chinese Journal of Biologicals

摘　　要：目的对无血清培养基与含血清培养基培养CHO细胞进行比较。方法应用两种培养基采用小方瓶培养CHO细胞,观察细胞维持时间、培养过程的形态变化及对血凝效价的影响。结果应用无血清培养基(A)培养CHO-C28细胞虽然可维持细胞正常生长,但贴壁不好,逐渐降低(A)加量情况可得到相应改善。结论 目前无血清培养基尚不能完全取代含血清培养基用于培养CHO细胞。Objective To evaluate the growth of CHO cells in serum-free and serum-containing media. Methods Culture CHO cells in serum-free and serum-containing media respectively, and compare the maintain time, morphological changes and hemagglutination liters of the cells in culture supernatant. Results The CHO-C28 cells cultured in serum-free medium grew well but showed no good adherence. However, along with the decrease of proportion of serum-free medium, the adherence improved. Conclusion At present, the serum-containing medium for culture of CHO cells can not be completely replaced by serum-free serum.

关 键 词：CH0细胞 血清 培养 培养基 生长 

分 类 号：Q813.11[生物学—生物工程]