蛋鸡血清与卵黄中禽流感病毒抗体的相关性  

Correlation between avian influenza virus antibody in serum of chickens and that in yolk

作　　者：黄建文[1] 毛跟年[2] 马秋明[1] 何维明[1] 朱旭东[1] 王霞 赵卓[1] 李联正[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]陕西科技大学生命科学与工程学院,陕西咸阳712000 [3]陕西咸阳渭城区畜牧兽医站,陕西咸阳712000

出　　处：《中国兽医科技》2004年第5期65-67,共3页Chinese Journal of Veterinary Science and Technology

基　　金：陕西省科技厅科技攻关项目 (99K0 6 G3) ;西北农林科技大学青年科研专项基金项目 (2 00 10 2 0 30 0 8)

摘　　要：用禽流感 (AI)H9N2油乳剂灭活疫苗免疫非免疫蛋鸡 ,并于免疫后第 0、3、5、7、9、11、13、15、17、19、2 1、30d分别测定血清及卵黄中的AI血凝抑制试验 (HI)及琼脂扩散凝集试验(AGP)抗体。结果显示 ,免疫后第 7d卵黄中即可检出HI及AGP抗体 ,以后逐渐上升 ,在免疫后第 19～ 2 1d达高峰值 ,与血清的HI抗体水平接近 ,差异不显著 (P >0 .0 5 )。表明通过对鸡蛋卵黄AIV抗体检测 ,可以进行AI的疫情监测 。In this study, non-vaccine layers were vaccined with the oil emulsion adjuvant inactive avian influenza(H9N2) vaccine. By means of using the haemagglutination inhibition (HI) test and the agar gel precipitation (AGP) test, antibodies in yolk and serum from the layers vaccined with the vaccine were (detected) on 0,1,3,5,7,9,11,15,17,19,21 and 30 day post-vaccination. Result showed that the antibody in yolk can be detected on 7 day post-vaccination, from then on, the correlation is more high, and that the top comes on from 19 day to 21 day post-vaccination. The mentioned-above result indicated that avian (influenza) plague can be monitored with the antibody in yolk to avian influenza virus.

关 键 词：蛋鸡 血清 卵黄 禽流感病毒 抗体 相关性 疫情监测 AI 

分 类 号：S858.315.3[农业科学—临床兽医学]