胰岛素样生长因子I(IGF-I)基因的克隆及分泌表达  

Gene Cloning and Secretory Expression of Human Insulin-like Growth Factor I(hIGF-I)

作　　者：赵大鹏[1] 王晓宇[1] 刘畅[1] 郭桥[1] 戚凤春[1] 蒙冲[1] 吴晓娟[1] 王妍[1] 盛君[1] 杨煜[2] 傅学奇[2] 

机构地区：[1]长春生物制品研究所,长春130062 [2]吉林大学,长春130021

出　　处：《中国生物制品学杂志》2004年第1期1-3,共3页Chinese Journal of Biologicals

基　　金：吉林省科技发展计划资助 ;吉科合字第 19980 5 77

摘　　要：目的　构建原核分泌型表达载体并高效表达胰岛素样生长因子I(IGF I)。方法　构建出 2种分泌型表达载体 ,命名为pCSA和pCST ,并将胰岛素样生长因子I(IGF I)基因插入pCSA和pCST中 ,转化E .coliW3110 ,经筛选获得工程菌pCSA/IGF I/W3110和pCST/IGF 1/W3110 ,并进行表达。结果　经SDS PAGE检测 ,在相对分子质量 76 0 0处有明显表达带 ,Westernblot表明重组蛋白具有IGF I的抗原性。结论　原核分泌型IGF I的高效表达 ,为进一步研究人IGFObjective To construct a prokaryotic vector for secretory expression of human insulin like growth factor I(hIGF I).Methods Construct 2 secretory expression vectors pCSA and pCST,then insert hIGF I into the 2 vectors and transform to E.coli W3110 respectively.Select recombinant strains pCSA/IGF I/W3110 and pCST/IGF W3110 for expression.Results SDS PAGE profile showed a clear protein band with a relative molecular weight of 7 600.Western blot proved that the recombinant protein had the antigenicity of IGF I.Conclusion hIGF I was successfully expressed in prokaryotic cells.It laid a foundation of further study on the function and biological properties of hIGF I.

关 键 词：胰岛素样生长因子I IGF-I 基因 克隆 分泌表达 质粒 

分 类 号：Q51[生物学—生物化学] Q78