具有中和活性抗呼吸道合胞病毒单克隆抗体的制备及鉴定  

Preparation and Characterization of Anti-Respiratory Syncytial Virus McAb with Neutralization Activity

作　　者：林林[1] 孟淑芳[1] 郎叔惠[1] 王佑春[1] 尹红章[1] 李德富[1] 

机构地区：[1]中国药品生物制品检定所,北京100050

出　　处：《中国生物制品学杂志》2004年第1期26-28,共3页Chinese Journal of Biologicals

摘　　要：目的　制备具有中和活性抗呼吸道合胞病毒的单克隆抗体。方法　采用杂交瘤技术制备单克隆抗体 ,并以ELISA结合免疫组化技术进行筛选。结果　获得 8株分泌抗呼吸道合胞病毒的特异性单克隆抗体的细胞株 ,其中 2株分泌的单抗具有中和活性 ,效价 1∶16 0和 1∶32 0。Objective To prepare anti respiratory syncytical virus McAbs with neutralization activity.Methods Prepare McAb by hybridoma technique,and screen by ELISA combined with immunohistochemical method.Results Eight cell strains secreting McAbs to respiratory syncytial virus were obtainad,of which 2 strains secreted McAb with neutralizing titers of 1∶160 and 1∶320 respectively.Conclusion The anti respiratory syncytical virus McAbs with neutralization activities were successfully prepared.

关 键 词：呼吸道合胞病毒 单克隆抗体 中和活性 杂交瘤技术 

分 类 号：R373.1[医药卫生—病原生物学] R392-33[医药卫生—基础医学]