Balb/c小鼠口服幽门螺杆菌疫苗rLTB-HspA的免疫应答  

Immune response of helicobacter pylori in Balb/c mice by oral immuneization with recombinant LTB-HspA

作　　者：郭鹰[1] 邹全明[1] 朱永红[1] 吴超[1] 郭刚[1] 毛旭虎[1] 袁小澎[1] 

机构地区：[1]第三军医大学医学检验系临床微生物学及免疫学教研室,重庆400038

出　　处：《免疫学杂志》2004年第3期201-203,207,共4页Immunological Journal

基　　金：国家"九五"重点科技攻关计划项目 (96 90 1 0 1 54);"863"生物与现代农业技术领域生物工程技术主题课题(2 0 0 1AA2 1 51 61 )资助

摘　　要：目的　研究幽门螺杆菌 (Hp)热休克蛋白A (rHspA)与大肠杆菌不耐热肠毒素B亚单位 (LTB)融合蛋白 (rLTB HspA)作为口服疫苗的免疫应答效果。方法　采用rLTB HspA口服免疫Balb c小鼠 ,ELISA分析小鼠血清、胃肠黏液中IgG、IgA抗体水平 ,体外培养脾淋巴细胞在Hp刺激下的增殖情况及IL 4、IFN γ的变化。结果　Balb c小鼠口服rLTB HspA能有效激发机体产生全身和局部特异性的体液免疫应答和细胞免疫应答。结论　Balb crLTBObjective To study the efficiency and immune response of recombinant LTB-HspA as an oral vaccine against in Balb/c mice. Methods Recombinant rLTB-HspA was used to immunize Balb/c mice by oral roufe. The specific antibodies IgG and IgA for helicobacter pylori were determined in serum, saliva feces and gastric juice with ELISA. Proliferation of spleen lymphocytes and the changes of IL-4, IFN-γ in the culture supernatant of spleen lymphocytes stimulated by helicobacter pylori were investingated with 3H-TdR Ruptaking and ELISA. Results The rLTB-HspA taken by Balb/c mice orally stimunated systematic and local immune response, humoral immune response, and cellular immune response. Conclusion The recombinant LTB-HspA may be used as vaccine to prevent helicobacter pylori infection.

关 键 词：幽门螺杆菌 大肠杆菌不耐热肠毒素B亚单位 热休克蛋白A 免疫应答 

分 类 号：R392[医药卫生—免疫学]