黑暗和光照对长春花培养细胞生长和生理生化特性的影响  

The Effects of Dark and Light on Growth and Physiological and Biochemical Characteristics in Cell Culture of Catharanthus roseus (L.)G.Don

作　　者：郭胜娟[1] 刘树楠[1] 冯玲玲[1] 周吉源[1] 

机构地区：[1]华中师范大学生命科学学院,武汉430079

出　　处：《武汉植物学研究》2004年第2期136-139,共4页Journal of Wuhan Botanical Research

摘　　要：以长春花(Catharanthusroseus(L.)G.Don)幼嫩叶片为外植体,在MS+NAA0.5mg/L+2,4-D0.5mg/L+6BA2.0mg/L培养基上诱导形成愈伤组织,愈伤组织置于不同条件下培养。结果表明,黑暗和光照下,培养细胞的生长周期为27d;过氧化物酶(POD)活性和超氧化物歧化酶(SOD)活性均出现2个峰值。对长春花总生物碱含量和总产量亦做了研究,结果发现最佳的收获时期为第27d。Based on young leaves of Catharanthus roseus (L.) G. Don, callus is induced on the solid medium of MS+NAA 0.5 mg/L +2,4-D 0.5 mg/L +6 BA 2.0 mg/L. Two groups of callus are cultured in the dark and light, respectively. The influeces of light on the cell growth and physiological and biochemical characteristics are studied. The results show that: both the cell growth curves are like 'S', and their growth circles are about 27 days. Activities of peroxides (POD) and superoxide dismutase (SOD) show two peaks. We also find that the alkaloid production increased during the growth circles and can get the most production at the 27th day.

关 键 词：愈伤组织 细胞生长 过氧化物酶 超氧化物歧化酶 生物碱 长春花 

分 类 号：Q945[生物学—植物学]