血浆蛋白对心血管生物材料细菌粘附影响的研究  

STUDY OF HUMAN PLASMA PROTEINS ON BACTERIAL ADHESION TO CARDIOVASCULAR BIOMATERIALS

作　　者：李艳星[1] 黄云超[1] 杨达宽[1] 张尔永[2] 石应康[2] 张敏 

机构地区：[1]昆明医学院第二附属医院胸心外科 [2]四川大学华西医院胸心外科 [3]四川大学华西医学中心基础同位素室

出　　处：《中国心血管病研究》2004年第5期375-379,共5页Chinese Journal of Cardiovascular Research

基　　金：该课题由云南省教委科研基金资助(99012206);云南省应用基础研究基金资助(2000C0110M);云南省中青年后备人才培养基金资助(2000YP10)

摘　　要：目的观察人体血浆蛋白对心血管生物材料细菌粘附的不同影响。筛选出抗细菌粘附力强的血浆蛋白预处理生物材料,为临床防治以生物材料为中心感染提供有效方法。方法 实验分5组,4个实验组和1个对照组。用3H-胸腺嘧啶脱氧核苷标记金黄色葡萄球菌(SA)、表皮葡萄球菌(SE),用四种血浆蛋白和生理盐水(对照组)预处理材料涤纶(Dacron)、膨体聚四氟乙稀(PTFE)。然后各组进行细菌与材料动态粘附实验。使用β-液闪测量仪(β—LSC),同时结合扫描电镜(SEM),观察实验材料上细菌粘附情况。结果 实验组与对照组比较,白蛋白能减少SA和SE对Dacron和PTFE的粘附(P<0.05);纤维蛋白原能增加SA对Dacron和PTFE的粘附,减少SE对Dacron粘附(P<0.05);纤雄连接蛋白增加sA对Dacron粘附,减少SE对Dacron和盯PTFE的粘附(P<0.05)。结论不同血浆蛋白对心血管生物材料细菌粘附有不同影响,有的血浆蛋白促进细菌粘附,有的抑制细菌粘附,适当的血浆蛋白预处理心血管生物材料,可能减少生物材料为中心感染(BCI)的发生率。Objective To observe the influence of human plasma proteins on staphylococcus aureus and staphylococcus epidermidin adhesion to cardiovascular biomaterials, Some plasma proteins of anti- bacterial adhesion were chosen to treat with cardiovascular biomaterials. It will provide an effective method for preventing the rate of Biomaterial Center Infect(BCI). Methods There were 5 groups in the experiment:4 tested groups,and 1 control group. Staphylococcus aureus and staphylococcus epidermidis were radiolabeled by 3 H - TDR and segments of Dacron and PTFE were preincubated for in plasma proteins and in normal saline and then the adhesive experiment of bacterial adhesion to Dacron and PTFE in vitro was performed. The radioactivity associated with segments was measured with Beta Liquid Scintillation Counting(β - LSC). Scanning electron microscopy(SEM) was used to observe the phenomenon of bacterial adhesion to biomaterials. Results Compared with control group, segments of Dacron and PTFE coated in Albumin can decrease the binding of Staphylococcus aureus and staphylococcus epidermidis both to Dacron and PTFE(P<0.05). Fibrinogen enhanced the binding of Staphylo-coccfus aureus both to Dacron and PTFE and decreased attachment of staphylococcrs epidemidis to Dacron(P<0.05). Fibronectin promoted the binding of Staphylococcrs aureus to Dacron and decreased attachment of staphylococcus epidermidis both to Dacron and PTFE(P<0.05). Conclusion The influence of various plasma proteins on bacterial adhesions to biomaterials and others can decrease bacterial adhesion. The special cardiovascular biomaterial coated in proper selected plasma proteins can decrease the rate of BCI in clinical.

关 键 词：血浆蛋白 心血管生物材料 细菌粘附 细菌数 

分 类 号：R54[医药卫生—心血管疾病] R318.08[医药卫生—内科学]