丝状霉形体丝状亚种SC型脂蛋白LppQN末端基因克隆与序列分析  

Cloning and Sequence Analysis for the N-terminal Domain of the Lipoprotein LppQ of Mycoplasma mycoide Subsp. Mycoides SC

作　　者：辛九庆[1] 高玉龙[1] 李媛[1] 王砚范[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所国家牛传染性胸膜肺炎参考实验室,黑龙江哈尔滨150001

出　　处：《中国兽医学报》2004年第3期252-254,共3页Chinese Journal of Veterinary Science

基　　金：国家"十五"科技攻关重大专项基金项目 ( 2 0 0 2 BA5 18A0 4)

摘　　要：脂蛋白 L pp Q是丝状霉形体丝状亚种 SC型 (Mm m SC)非洲株、欧洲株和疫苗株所特有的。 L pp Q N末端域具有良好的免疫原性 ,在牛体内可诱导产生强大、特异、早期、持续的免疫反应。本研究根据已发表的 L pp Q基因序列设计引物 ,用 Pyrobest TM高保真 DNA聚合酶从 Mmm SC HVRI X株中扩增出了 L pp Q N末端基因序列 ,并进行了克隆与序列测定。核苷酸序列比较结果显示 ,HVRI X株的 L pp Q N末端基因序列与国外发表的序列同源性为 99.7% ,由其推导的氨基酸序列同源性为 99.1% ,为脂蛋白 L pp QThe lipoprotein LppQ gene is specific to M.mycoides subsp.mycoides SC(MmmSC) and was found in field strains isolated in Europe,Africa,as well as in vaccinal strains.The LppQ N-terminal domain possess the strong immunogenicity and induce a strong,specific,early,and persistent immune response in naturally and experimentally infected cattle.The LppQ N-terminal gene was amplified from MmmSC HVRI X strain by PCR using the primer pair designed according to the reported MmmSC LppQ sequence.It was cloned and sequenced subsequently.The result revealed that there was 99.7% identical in nucleotide sequence,99.1% identical in induced amino acid sequence,respectively.Molecular cloning of MmmSC LppQ N-terminal gene makes it possible to express the LppQ N-terminal gene in vitro.

关 键 词：牛传染性胸膜肺炎 丝状霉形体丝状亚种SC型 LppQ N末端 序列分析 基因 克隆 

分 类 号：S852.62[农业科学—基础兽医学] S858.235[农业科学—兽医学]