水飞蓟宾-磷脂酰胆碱复合物对四氯化碳致小鼠急性肝损伤的保护作用  

Protective effects of silybin-phosphatidylcholine compound on acute liver damage in mice induced by CCl_4

作　　者：陈正跃[1] 吴子钊[2] 张光军[2] 许建文[2] 程钢[2] 

机构地区：[1]华中科技大学同济医学院病理生理学系,武汉430030 [2]新乡医学院药物研究室,新乡453003

出　　处：《中国新药杂志》2004年第4期319-321,共3页Chinese Journal of New Drugs

基　　金：河南省科技攻关项目(991180204)

摘　　要：目的:研究水飞蓟宾-磷脂酰胆碱复合物(SPC)对四氯化碳(CCl4)所致的小鼠急性肝损伤的预防和治疗作用。方法:50只小鼠随机分成5组,每组10只:正常对照组、模型组、预防组、治疗组和预防+治疗组,采用CCl4造成小鼠急性肝损伤模型,预防组于注射CCl4前多次给小鼠灌服SPC,治疗组于注射CCl4后给SPC,预防+治疗组于注射CCl4前后均给SPC,24h后采血,分离血清,检测血清ALT和AST活性,并取肝组织观察比较肝脏病理组织学改变。结果:SPC明显降低小鼠血清ALT和AST活性,治疗、预防及预防+治疗组与模型组比较差异均有极显著性(P<0.01),预防组与治疗组比较差异有显著性(P<0.05),预防+治疗组与治疗组比较差异有极显著性(P<0.01),且均能改善肝脏病理组织学变化。结论:SPC对CCl4造成的急性肝损伤有明显的预防和治疗作用。Objective: To study the protective and therapeutic effects of silybin-phos-phatidylcholine compound (SPC) on acute liver damage in mice induced by CC14. Methods: 50 mice with CCl4 induced acute liver damage were randomly and equally divided into 5 groups: normal group, model group, protective group, therapeutic group and protective plus therapeutic group. In protective group,mice were received multi doses of oral SPC prior injection of CCl4. In therapeutic group, mice were received SPC after injection of CC14. In protective plus therapeutic group,mice were received SPC prior and after injection of CCl4 .24h later,blood were taken from mice of all groups to determine the serum level of ALT and AST and livers were taken out to observe histopathological changes. Results: In comparison with model group, the serum AST and ALT level in mice of other 3 groups were-significantly decreased (P<0.01) by SPC therapy. There was significant difference between protective group and therapeutic group (P < 0. 05), and greatest significant difference was existed between protective group and protective plus therapeutic group (P<0.01). Liver histopathological improvement was observed in other 3 groups in comparison with model. Conclusion: SPC is highly effective in protection and treatment of acute liver damage in mice induced by CCl4.

关 键 词：水飞蓟宾-磷脂酰胆碱复合物 四氯化碳 肝损伤 保护作用 

分 类 号：R975.5[医药卫生—药品] R965.1[医药卫生—药学]