溶藻弧菌内毒素免疫Balb/c小鼠方法的研究  

IMMUNIZATION OF BALB/C MICE WITH ENDOTOXIN FROM VIBRIO ALGINOLYTICUS

作　　者：陈晓燕[1] 胡超群[1] 

机构地区：[1]中国科学院南海海洋研究所,广州510301

出　　处：《热带海洋学报》2004年第3期6-10,共5页Journal of Tropical Oceanography

基　　金：国家重点基础研究发展规划项目(G1999012003);中国科学院知识创新工程项目(KSCX2-SW-302-7)

摘　　要：采用热酚水法提取点带石斑鱼(Epinephelus malabaricus)病原溶藻弧菌VAL02菌株的内毒素脂多糖(lipopolysaccharide,LPS)。用提取的LPS按15、30、60μg·只-1和VAL02菌株颗粒抗原按1×108(不加佐剂)和1×108cell·只-1。(加佐剂)的方式免疫Balb/c小鼠,经1次基础免疫和6次加强免疫后,间接ELISA检测。结果表明颗粒抗原加佐剂免疫组效果最好。An endotoxin (lipopolysaccharide,LPS) was extracted from Vibrio alginolyti-cus , which is a pathogen of Epinephelus malabaricus , by hot phenol-water method. Balb/c mice were immunised by 15μg/dose, 30μg/dose, 60μg/dose of extracted LPS, and by 1×108cell/dose of formalin-killed bacterial cells with or without Freund' adju-vant. The anti-LPS IgG level in mouse serum can be efficiently raised by 7 injections of formalized bacterial cells emulsified with adjuvant.

关 键 词：溶藻弧菌(Vibrio alginolyticus) 内毒素 免疫 

分 类 号：S942.5[农业科学—水产养殖]