银杏酸的分光光度法测定  

Determination of Ginkgolic Acids by Ultraviolet Spectrophotometry

作　　者：仰榴青[1] 吴向阳[2] 陈钧[3] 袁新华[2] 倪慧艳[1] 

机构地区：[1]江苏大学药学院,镇江212001 [2]江苏大学化学化工学院,镇江212013 [3]江苏大学生物与环境工程学院,镇江212013

出　　处：《分析化学》2004年第5期661-664,共4页Chinese Journal of Analytical Chemistry

基　　金：江苏省高校自然科学研究基金资助项目 (No .0 1KJD3 5 0 0 0 1)

摘　　要：建立了简便、快速、准确的银杏酸含量的分光光度分析方法。银杏酸粗提物经硅胶柱色谱一次预净化处理后 ,即可在 2 4 2nm或 310nm波长处测定银杏酸。线性回归方程分别为 :A =1.5 5× 10 -4+16 .36C(R =0 .999)或A =0 .0 31+9.0 8C(R =0 .997) ,两者的线性范围均为 6 .0～ 110mg/L。在 2 4 2nm和 310nm两个波长处测定的银杏酸含量都与HPLC法测定结果接近。实验测得了各银杏酸单体的摩尔吸光系数 ,其数值相近。该法简便、快速、准确 ,线性范围宽 ,最低可测至 5 .72mg/L 。A simple, fast and accurate analytical method for the determination of ginkgolic acids by ultraviolet (UV) spectrophotometry was established. The content of ginkgolic acids in crude extract can be determined directly by UV spectrophotometry at 242 nm or 310 nm after one-pre-purified-step by silica gel column chromatography. The linear regression equations were A = 1.55 x 10(-4) + 16.36C(R =0.999) and A =0.031 +9. 08C (R = 0.997) respectively. The linear ranges of the two wavelengths for the determination were 6.0 similar to 110 mg/L. The results being detected at both wavelength of 242 nm and 310 nm by UV spectrophotometry were close to those by high performance liquid chromatography. The molar absorption coefficients of ginkgolic acids were also determined, and they were approach. Not only is the method found to be simple, fast and accurate, but also has wide linear scope. The limit of detection is 5.72 mg/L. The method can be used for the determination of ginkgolic acids.

关 键 词：银杏酸 分光光度法 测定方法 毒性物质 银杏外种皮 银杏叶 药物 

分 类 号：O657.3[理学—分析化学]